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Structures and biological functions of zinc finger proteins and their roles in hepatocellular carcinoma

Biomarker Research volume 10, Article number: 2 (2022) Cite this article

Abstract

Zinc finger proteins are transcription factors with the finger domain, which plays a significant role in gene regulation. As the largest family of transcription factors in the human genome, zinc finger (ZNF) proteins are characterized by their different DNA binding motifs, such as C2H2 and Gag knuckle. Different kinds of zinc finger motifs exhibit a wide variety of biological functions. Zinc finger proteins have been reported in various diseases, especially in several cancers. Hepatocellular carcinoma (HCC) is the third leading cause of cancer-associated death worldwide, especially in China. Most of HCC patients have suffered from hepatitis B virus (HBV) and hepatitis C virus (HCV) injection for a long time. Although the surgical operation of HCC has been extremely developed, the prognosis of HCC is still very poor, and the underlying mechanisms in HCC tumorigenesis are still not completely understood. Here, we summarize multiple functions and recent research of zinc finger proteins in HCC tumorigenesis and progression. We also discuss the significance of zinc finger proteins in HCC diagnosis and prognostic evaluation.

Background

Zinc finger proteins (ZFPs), which constitute the largest transcription factor family with finger-like DNA binding do- mains, play a significant role in multiple biological processes. ZFPs primarily function as transcription factors in tumorigenesis and tumor progression. Transcription factors (TFs) are proteins that play a vital role in complicated biological processes, such as metabolism, autophagy, apoptosis, immune responses, stemness maintenance and differentiation. TFs regulate transcription of genes by recognizing or binding to DNA sequences directly [1, 2]. So far, zinc finger motifs have been classified into eight different categories according to their main-chain conformation and secondary structure around their zinc-binding sites, including Cys2His2 (C2H2) like, Zn2/Cys6, Treble clef, Zinc ribbon, Gag knuckle, TAZ2 domain like, Zinc binding loops and Metallothionein [3, 4]. In addition to these zinc motifs, ZFPs also contain several domains that play different roles in cell biological processes, including BTB (Broad-Complex, Tramtrack, and Bric-a-brac), the Krüppel-Associated Box (KRAB) domain, SET domain and SCAN (SRE-ZBP, CTfin51, AW-1 and Number 18 cDNA) domain. Because of the diversity of zinc finger motifs and these domains, ZFPs can play different roles in gene regulation under various cellular environments and other stimuli.

Hepatocellular carcinoma (HCC) accounts for a major part of the global cancer category. It accounts for approximately 75–80% of primary liver cancer [5, 6]. According to the Global online database of infectious diseases analysis, the incidence of liver cancer decreased in many Asian countries during 1978 ~ 2012. Still, it rebounded in India, the United States and other European countries [6]. Since patients have no obvious clinical symptoms in the early stage of HCC, the early diagnosis of HCC becomes extremely difficult. Although there are many treatments, including surgical resection, chemotherapy, targeted therapy, the prognosis of liver cancer is still not optimistic. However, the numerous molecular mechanisms in HCC pathogenesis remain unclear. In recent years, studies about ZFPs functions in multiple cancers have been constantly emerging [7,8,9,10,11,12,13,14]. These studies offered promising treatments for malignancies, including HCC [15]. In this review, we will discuss ZFPs basic spatial structures and the complex mechanisms of ZFPs in HCC.

Structures of zinc finger proteins

BTB domain

The BTB domain (also known as the POZ domain) is a multifaceted protein-protein interaction motif found in whole eukaryotes. At the same time, it has been reported in previous literature that some poxvirus proteins are similar to a part of ZFPs, like ZID, GagA and ZF5; this domain has been named as POZ (poxvirus and zinc finger) domain [16, 17]. As a highly conserved structure, it engaged in multiple cellular functions, including transcription repression, cytoskeleton dynamics, tetramerization and gating of ion channels, and targeting proteins for ubiquitination [18,19,20,21,22,23]. The BTB domain consists of a cluster of five α-helixes, one end covered by a short three-chain β-fold, which is compact and spherical [24]. In different BTB domains, their primary structure is less conservative, but their secondary structure is broadly similar. It has been reported that the BTB domain can be classified into four families: T1, Skp1, ElonginC, and BTB-ZF, but the details have not yet been worked out. Interestingly, the BTB domain in ZFPs can be homologous and heterogenous, or the BTB domain can recruit co-inhibitors of transcription. The function of proteins containing the BTB domain can be roughly divided into two categories: transcriptional inhibition and protein degradation, which are crucial for genes to function in cell development [25,26,27]..

SCAN domain

Scan domain, a highly conservative domain, consists of 84 amino acids rich in leucine residues, is also known as the leucine-rich region [28, 29]. To date, about 244 protein products containing the scan domain have been identified in the human genome, of which about 50 scan-containing transcription factors have been described. The scan domain has an amphipathic secondary structure that participates in protein-protein interaction, especially self-binding and mediated oligomerization [25, 30, 31]. Further studies of the domain have shown that it can interact with separated scan domains, such as scan domain protein 1 (SDP1), or with other family members with scan domains [32]. It is worth mentioning that this interaction is not universal but selective, suggesting that not all family members can form oligomerization. Interactions between different scan-containing transcription factors lead to various transcription activities. In addition, few proteins may have several special names depending on their domain. As can be seen from the NCBI gene database, the human ZSCAN (zinc finger and scan) transcription factor family members have a uniform name ranging from ZSCAN1 to ZSCAN54 [33].

KRAB domain

Krüppel-associated box (KRAB) domain-containing zinc finger proteins (KZFPs), which have been reported to exist only in quadrupeds. The human genome encodes approximately more than 350 KZFPS. In recent reports, KZFPs mainly inhibit transposable elements (TEs) by recruiting transcriptional regulators and heterochromatin formation and DNA methylation in embryonic stem (ES) cells [34, 35]. The KRAB domain consists of 75 amino acids, and the domain is usually disconnected into two adjoining modules: A-box, which is mainly responsible for inhibiting activity by interacting with corepressors; and B-box, which is believed to augment the ability of repression of A-box through some undiscovered mechanisms [36,37,38]. Theoretically, the length of KRAB-ZFP should be enough to recognize longer DNA target sequences specifically, but in fact, the binding motif of KRAB-ZFP is often shorter than predicted. This implied that in KRAB-ZFP, different ZNF recognize different DNA motifs [39]. In addition, ZNF is not only involved in contacting with DNA but also can participate in other categorizes of interactions, such as interactions with RNA, or proteins [40,41,42,43]. A few ZFPs contain added domains, including BTB domain, SCAN domain, KRAB domain, SET domain, DUF3669 domain and C2H2 motif. Regrettably, the precise function of the DUF3669 domain is still unclear (Fig. 1).

Fig. 1
figure 1

Several domain structures of C2H2-ZFPs. Three diverse forms of C2H2-ZFPs are described. Each C2H2-ZFP contains at least one KRAB structural domain, BTB domain, SCAN domain, SET domain and several zinc fingers which can bind to DNA sequences. In brief, the KRAB domain can be divided into two parts: the A-box (KRAB-A) and the B-box (KRAB-B). As shown in type 4, some C2H2-ZFPs contain a DUF3669 domain. Functionally, BTB domain is mainly responsible for transcriptional repression and protein degradation; SCAN domain is mainly responsible for protein binding and protein oligomerization; KRAB domain is mainly responsible for repression of transposable elements; SET domain is mainly responsible for protein methylation (mainly histones); C2H2 motif can bind to DNA, RNA, and proteins to perform different functions, but most of them bind to DNA.ZF: zinc finger; BTB: Broad-Complex, Tramtrack, and Bric-a-brac. KRAB:Krüppel-associated box; SCAN: SRE-ZBP, CTfin51, AW-1, and Number 18 cDNA; DUF3669:domain of unknown function 3669

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SET domain

The SET structural domain is widely found in eukaryotes and consists of approximately 130 amino acids and is named after the Drosophila proteins Suppressor of variegation 3–9 (Su (var)3–9), Enhancer of zeste (E(z)), and Trithorax (Trx). The biological behaviours in which the SET structural domain is involved are primarily associated with the methylation of substrates. For example, many SET domain-containing proteins can mono-, di- or trimethylation of their lysine substrates using the cofactor S-adenosyl-L-methionine (SAM), these catalyzed targets include histones and many non-histone substrates. In addition, the SET domain does not usually exist as a stand-alone entity, and in many proteins, it co-exists with other protein structural domains and interacts with proteins that regulate its catalytic function [44, 45].

C2H2-zinc finger motif

Among eight different groups of zinc fingers, the most classical zinc finger is the C2H2-like finger [3]. As the most prevalent motif in ZFPs, over 5000 C2H2-like fingers have been encoded by the human genome [46]. The zinc finger structure is generally located at the C-terminus of the ZFPs. The consensus sequence for C2H2 zinc fingers is CX2CX3FX5LX2HX3H, which contains conservative hydrophobic residues wrapped in hydrophobic cores except for its two cysteines and two histidine residues. These conservative amino acids and a characteristic structure is consisting of a two-stranded antiparallel β-sheet and an α-helix [47]. It has been suggested in the article that an individual zinc finger motif binds an adjacent three-nucleotide subsequence, maybe three to five, and a C2H2-zinc finger domain can be specified to a range of three base pair (3 bp) targets [48, 49].

C2H2-type ZFPs generally contain added anatomic domains, such as BTB (Broad-Complex, Tramtrack, and Bric-a-brac), the Krüppel-Associated Box (KRAB) domain, SET domain and SCAN (SRE-ZBP, CTfin51, AW-1 and Number 18 cDNA) domain [40]. These structures regulate immune response, cell differentiation and embryonic development at the transcription and translation level through specifically binding to the target molecule DNA, RNA, DNA-RNA sequence, and binding to itself or other ZFPs [48]. For instance, ZEB1 can actuate transcription through binding to coactivators, such as p300 and P/CAF [50, 51].

Comparison of the functions between these structural domains

The domains of ZFPs are not only diverse, but the functions of these domains are also very distinct. For example, although both the BTB domain and the SCAN domain can bind to the protein, but the BTB domain mostly inhibits the transcriptional activity of the target gene by recruiting transcription co-repressors, while the SCAN domain causes oligomerization of target proteins through its specific binding [27, 31]. In addition, KZFPs can inhibit transposable elements through DNA methylation, while SET domains that have different substrates are through protein methylation to play its catalytic role [34, 44]. In a word, various C2H2-type ZFPs contain different types of the above-mentioned domains, and these ZFPs with different functions play a significant role in in cell biological processes.

Biological functions of zinc finger proteins

Among the numerous biological functions of ZFPs, transcription regulation occupies a highly prominent position. ZFP relies on the domain-containing zinc finger in one segment to bind to the promoter region of the target gene and act as a transcription enhancer or transcription inhibitor. In addition to transcriptional regulation, ZFPs can induce protein-protein interactions, bind to RNA, and interact with proteins and RNAs in the meantime [52,53,54,55].

Transcription regulation

As the most prominent role of ZFPs, the functions of transcriptional regulation are mainly reflected in both transcriptional promotion and transcriptional repression. It has been recently reported that ZEB1 (Zinc finger E-box binding homeobox 1) can bind to the HDGF (hepatoma-derived growth factor) promoter to stimulate HDGF transcription in EC (endometrial carcinoma) cells [56]. Another example on the contrary, ZNF322A, a C2H2 zinc finger transcription factor, can directly bind and recruit histone deacetylation enzyme 3 to the c-Myc promoter to suppress c-Myc expression at the level of transcription. Then increase mitochondrial phosphorylation to promote cell movement and ultimately maintain stem cell-like characteristics in lung cancer [57].

Protein interactions

As well as functioning as transcription factors, numbers of ZFPs that can induce protein interactions have also been identified in recent years [58]. For example, the striated muscle RING zinc finger protein (SMRZ) is a novel human striated muscle ZFP which has a ring domain at its N-terminal. In recent studies, the SMRZ was reported to interact with SMT3b, which was a ubiquitin-like protein, and their interactions are completed by the ring domain of SMRZ. This kind of interaction may contribute to the regulation of cell cycle that occurs during the process of growth in striated muscle cells [59].

Post-transcriptional regulation

Some ZFPs also have RNA binding properties, and these ZFPs are generally thought to be involved in post-transcriptional regulatory processes such as mRNA maturation splicing and degradation [60, 61]. Recently, the zinc finger protein Regnase-1, which had been revealed to regulate self-renewal of HSPCs (Hematopoietic Stem and Progenitor Cells) through modulating mRNA stability. More specifically, Regnase-1 could degrade the mRNAs of Gata2 and Tal1 by targeting the 3’UTR region of their mRNAs, and caused a decrease in the expression of GATA2 and Tal1, which could ultimately lead to a slowdown in the self-renewal of HSPCs and affected the homeostasis of HSPCs [62].

Effects of ZFPs via different mechanisms

Lipid metabolism

An increasing number of studies have shown that metabolic manipulate and signaling pathways are closely linked, not only in normal cells but also in cancer cells [63]. Recently, Li et al. suggested that YY1 prevented proliferator-activated receptor gamma coactivator-1β (PGC-1β) expression by directly binding to its promoter, thereby inhibiting the oxidation of fatty acid β, which led to the accumulation of lipid in HCC cells and induced the carcinogenic potentiality of HCC cells. It was worth mentioning that the reduction of the PGC-1β expression level by YY1 was independent of HIF-1α (hypoxia-inducible factor-1α) expression. This provided us with new insights into the conditions under which HIF-1α works [64,65,66]. Liu et al. revealed that ZBTB20, a regulator of lipid homeostasis, promoted hepatic de novo lipogenesis (DNL) by directly binding to and enhancing the activity of the ChREBP-α promoter and indirectly activating ChREBP-β, which provided new insights into the transcriptional regulatory network of DNL and had the potential to be a target for the treatment of fatty liver disease (FLD) [67].

Cell differentiation

ZFPs like Snails (Snai1, Snai2/Slug, Snai3/Smuc) and the E-box binding proteins ZEBs (ZEB1, ZEB2) can also affect the process of cell differentiation directly or indirectly [68]. Goossens et al. demonstrated that ZEB2 had high mRNA levels in hematopoietic stem cells and hematopoietic progenitor cells (HPCs). Sophisticated cellular analysis showed that ZEB2 was imperative for normal HSC/HPC differentiation, and ZEB2 deficient HSCs/HPCs were not properly implanted in fetal liver or bone marrow, and exhibited boosted adhesion associated with upregulated expression of beta1 integrator and CXCR4 [69,70,71,72,73,74]. Tang et al. expounded that Snail and Slug could cooperatively control skeletal stem/stromal cell (SSC) self-renewal in knockout mouse models. Mechanistically, Snail/Slug were reported to regulate SSC function by forming a complex with transcriptional co-activators YAP and TAZ, thereby inhibiting the Hippo pathway-dependent YAP/TAZ signaling cascade regulation. The regulatory network within the above mechanism provides new thought on cell differentiation in normal hepatocytes and HCC cells [75]. In addition, snail can also be involved in regulating cell stemness in glioma stem cells (GSCs). A recent study revealed that snail could inhibit TGFβ1 transcription and reduce its activity through a positive feedback loop resulting from its interaction with SMAD. This process contributes to regulation of the opposite BMP and TGFβ pathway activity, thereby inhibiting GSC stemness ultimately [76].

Immune response

Few ZFPs have recently been reported in immune-related processes such as immune response, immune homeostasis, and cytokine production in emerged new studies [77, 78].

Gfi1, which function as a transcriptional repressor, is a zinc finger protein that participates in diverse development contexts. Jin et al. demonstrated that Gfi1 (growth factor independent 1 transcriptional repressor) played a limiting role in the inflammatory response induced by endotoxin in mouse lung, and its regulatory function in alveolar macrophages as downstream of LPS (Lipopolysaccharide) receptor (TLR4) and upstream of TNF further elucidated the mechanism of Gfi1’s role in the endotoxin response [79]. By the way, the toll-like receptor (TLR) family plays a directive role in inflammatory response, antiviral, and activation of transcription factors [80]. Moreover, ZFYVE1 (zinc finger FYVE-type containing 1) can bind to poly(I:C) and TLR3 (Toll-like receptor 3) through the FYVE structural domain and enhance its association with TLR3 in response to poly(I:C) stimulation. Subsequent overexpression of ZFYVE1 can also significantly promote the binding of TLR3 to its ligand poly(I:C). Although the absence of ZFYVE1 can inhibit TLR3-mediated innate immune and inflammatory responses, it cannot inhibit TLR4-mediated the same reactions as TLR3 [81]. The above roles of these ZFPs in innate immunity and the associated inflammatory response provide deep thinking for subsequent studies.

CCCH zinc finger proteins consist of several CCCH zinc finger domains, which are composed of one histidine and three cysteines. A small number of CCCH zinc finger proteins have been reported to play important roles in immune responses [82, 83]. For instance, TTP (aka ZFP36), roquin 1 and MCPIP1, can form a regulatory network that maintains immune homeostasis [84,85,86]. The regulatory network composed of these three ZFPs accelerates the regression of inflammation, manage the sizes and rhythms of the adaptive immune response, targets mRNA to regulate its half-life and regulates signal pathways [87, 88]. However, based on the characteristics of this type of ZFP that can shuttle between different cell compartments, there is still a lot to be studied, especially in cell metabolism and cellular immunity [89].

Zinc finger proteins in HCC

In the past decades, the role of ZFPs has been reported in various of cancers including nasopharynx, esophagus, lung, gastric, colorectal, breast, thyroid, prostate, ovarian cancer [57, 90,91,92,93,94,95,96,97]. However, the number of studies in HCC is much less than other cancer types, and there are very few systematic reviews about HCC [98,99,100,101]. To provide a better understanding of ZFPs in HCC and explore the potential therapeutic targets. Here, we firstly summarize the functions of ZFPs in HCC and the specific mechanisms (Tables 1 and 2), and some specific examples are shown in the form of figure (Fig. 2).

Table 1 Oncogenic role of ZFPs in HCC
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Table 2 Tumor-suppressive roles of ZFPs in HCC
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Fig. 2
figure 2

Zinc finger proteins influence hepatocarcinogenesis through different ways. A: ZNF384 activates Cyclin D1 transcription by directly binding to its promoter, and facilitates G1/S phase transition, ultimately promotes proliferation of HCC. B: ZNF703 promotes HCC metastasis and induces through transactivating CLDN4 expression. C: A20 decreases the protein level of PFKL by promoting ubiquitination and degradation of PFKL, then inhibits progression of HCC through downregulating glycolysis

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Cell cycle

The cell cycle regulation cannot be separated from cyclins, CDKs (Cyclin-dependent kinases) and CKIs (Cyclin-dependent kinase inhibitors) [114]. CDC6 (cell division cycle 6) is a protein essential for the initiation of DNA replication [102]. The overexpression of ZNF143 facilitated HCC cell cycle progression via activating CDC6. Concretely, ZNF143 was reported to directly activate transcription of histone demethylase mineral dust-induced gene (MDIG) and decreased the enrichment of H3K9me3 in the CDC6 promoter region [115]. In addition, Cyclin D1 could form a complex with CDK4 or CDK6, whose activity was required for cell cycle progression [116]. He et al. demonstrated that knockdown of ZNF384 could result in sluggish of the G1/S phase transition in HCC. Mechanistically, ZNF384 was found to upregulate Cyclin D1 by binding to its promoter region, then accelerated G1/S phase transition and promotes proliferation of HCC [110].

Apoptosis

The process of apoptosis is induced by two distinct pathways: the intracellular pathway or the extracellular pathway [117]. In the extracellular pathway, apoptosis begins with binding a family of death receptors to an appropriate ligand on the membrane, then the death-inducing signaling complex (DISC) is recruited and activated, which consists of FADD (Fas-Associated protein with Death Domain), the death-executing protease enzyme caspase-8 and FLIP [118]. Liang et al. revealed that overexpression of ZNF307 upregulated the protein level of caspase-3 and Bax (BCL2 associated X), while decreased the protein level of Bcl-2 (BCL2 apoptosis regulator). It was the first time that ZNF307 was reported to function as tumor suppressor in HCC by inducing apoptosis by targeting these genes [103]. It was worth mentioning that Bax and Bcl-2 is also regulated by other ZFP in HCC. In a previous study, GLI1 was aberrantly overexpressed in HCC [119]. PCAF modulated the GLI1/Bcl-2/BAX axis to induce apoptosis in HCC. Gai et al. suggested that the cytoplasmic GLI1 protein was acetylated directly at lysine 518 by PCAF (P300/CBP-associated factor), while the nuclear translocation and promoter occupancy of GLI1 were prevented. Subsequently, the expression of Bcl-2 was decreased, and BAX was increased [120]. Additionally, ZNF263 could promote resistance to apoptosis in HCC indirectly by activating ER stress-dependent autophagy, and the specific mechanism has not been further investigated [121].

Stemness maintenance

Cancer stem cells (CSCs) are responsible for self-renewal, maintenance, and the growth of tumors [122]. The ability of CSCs to evade cell death and metastasis is significant for tumorigenesis [107, 123]. Zinc finger protein X-linked (ZFX), which was highly conserved in vertebrates. According to previous studies, ZFX was concerned with the initiation or progression in various of human cancers [124, 125]. Recently, Lai et al. demonstrated that overexpression of ZFX leaded to the upregulation of Nanog and SOX-2, which could play a vital role in the development of embryonic stem cells (ESCs) [126]. Mechanistically, ZFX could bind to the promoter of Nanog and SOX-2 directly and activate their expression, then contributed to the maintenance of stem-like characteristics of HCC cells [127]. Similarly, ZFX could also upregulate the expression of epithelial cell adhesion molecule (EpCAM) in HCC. Specifically, knocking-down of ZFX could inhibit CSCs-associated gene expression, self-renewal capacity, metastatic potential and tumorigenicity. Depletion of ZFX was demonstrated to reduce the transactivation and nuclear translocation of β-catenin and maintained the stemness of liver CSCs by activating β-catenin. The expression level of ZFX and EpCAM could be a significant prognostic factor of patients in HCC [128].

EMT and metastasis

The epithelial to mesenchymal transition (EMT) plays a meaningful role in the early steps of metastasis of tumor [104]. In a previous study, TGF-β had been identified as one of the most potential inducer of EMT, which could play a dual role by performing an anti-oncogenic effect at the early stages of tumor and being oncogenic at later stages [109, 129]. As one of the classic EMT activators, ZEB1 plays a significant role in HCC. Li et al. suggested that ZEB1 markedly enhanced the Wnt signaling pathway, promoted the proliferation and migration of HCC, and this phenomenon could be abolished by miR-708 [130]. This regulatory mechanism might provide a new therapeutic target for the treatment of HCC. In addition, Snail, another classic transcription factor, is also involved in the induction of EMT. For example, Jiao et al. demonstrated that Snail and E-cadherin are negatively correlated with mRNA and protein levels in HCC cells, and the high expression level of Snail in HCC often indicates a poor prognosis [131]. Recently, Wang et al. revealed that ZNF703 overexpression could promote HCC metastasis and sorafenib resistance by regulating EMT via upregulating CLDN4. Precisely, ZNF703 activated CLDN4 by binding directly to promoter of CLDN4. Moreover, the Kaplan–Meier analysis showed that ZNF703 could be considered as an indicator for predicting the prognosis of patients with HCC [106]. GATA4 was shown as a ZFP that was identified as a regulator of cardiac development and adult cardiac hypertrophy [132]. Overexpression of GATA4 could lead to the upregulation of E-cadherin and the downregulation of N-cadherin and vimentin, which were vital markers in the EMT process. This phenomenon could result in the mesenchymal-to-epithelial transition of HCC cells, but the specific mechanism was still unknown [133]. ZNF503 was reported to function as a transcriptional repressor in breast cancer and increase mammary epithelial cell proliferation [112, 134]. Recently, the role of ZNF503 in the development of HCC and tumor initiation was uncovered. Yin et al. suggested that mRNA level and protein level of ZNF503 were upregulated in HCC tissues and cell lines. Additionally, ZNF503 could promotes invasion, migration, and EMT processes in HCC. Mechanistically, ZNF503 was demonstrated to be recruited to promoter of GATA4, then represses its expression, which played a reverse role of ZNF503 [135].

Metabolism reprogramming and glucose metabolism

Cellular metabolism functions as a flexible network not only in normal tissues but also in the development of malignancies [136]. Metabolic reprogramming is the process that tumor cells reprogram the acquisition and metabolism of nutrients to meet their needs for energy, protein synthesis, and maintenance of redox homeostasis [137]. In the past decades, ZFP91 had been reported to be associated with inflammatory response but rarely studied in the metabolism reprogramming [138]. Recently, Chen et al. demonstrated that ZFP91 could inhibit hnRNP A1-dependent PKM splicing and ultimately suppress glucose metabolism reprogramming, cell proliferation and metastasis of HCC. Specifically, overexpression of ZFP91 could promote the Lys48-linked ubiquitination of hnRNP A1 at lysine 8 and proteasomal degradation, which could block the process of hnRNP A1-dependent PKM splicing, and result in the downregulation of PKM2 (Pyruvate kinase M2) and the upregulation of PKM1 (Pyruvate kinase M1) [139]. The formation of PKM2 is critical in the Warburg effect in cancer cells [108]. The Warburg effect causes an increase in glucose uptake and lactate production, which provides a selective advantage for tumor progression [140]. From the perspective of treatment, blocking up the ZFP91-hnRNP A1 pathways may be an effective method in the future.

A20, also known as tumor necrosis factorα-induced protein 3 (TNFAIP3), is an E3 ubiquitin ligase containing ring finger domains and is also a hotpot in immunoregulation [113, 141, 142]. Recently, A20 was found to correlate with glucose metabolism. Feng et al. revealed that A20 could interact with PFKL (phosphofructokinase, liver type) and promote its ubiquitination and degradation, thus inhibiting glycolysis in HCC cell lines and ultimately inhibiting proliferation, migration, and glycolysis of HCC [143]. PFK (phosphofructokinase) was found as the most important rate-limiting enzyme in glycolysis, and PFKL was one of its isoforms in the human liver [144, 145]. This study filled the gap in the glycolytic pathway and provided possible therapeutic targets for HCC treatment.

Regulation of inflammation

Liver is the main organ where immune response and immune tolerance occur, and many immune reactions in normal hepatocytes and HCC cells may impact on the immune function of the liver [146]. Recently, several researches showed that ZFPs could influence important process of immune responses. Fortunately, Zhang et al. suggested that the ZFP Miz1 could suppress liver tumorigenesis by restraining hepatocyte-driven macrophage activation and inflammation [105, 147]. On the one hand, the cytosolic Miz1 could interfere with the interaction between MTDH and RelA through competitive binding, thereby limiting DEN/ccl4-induced activation of NF-kB in hepatocytes in HCC. Additionally, Miz1 could inhibit MTDH phosphorylation by IKK (the IkB kinase complex), which was an activator of NF-kB after activated by varies extracellular stimuli, then inhibiting NF-kB transcriptional activity [111]. On the other hand, hepatocyte-specific Miz1 decrease produced a unique hepatocytes subset with the up-regulation level of TNF-α, IL-1b, IL-6, and CCL4, which promoted tumor-infiltrating macrophages to a pro-inflammatory phenotype, thereby promoting an inflammatory response in HCC.

ZNF191: double-edged sword in HCC

In most conditions, ZFPs tend to play only one of the two effects, anti-oncogenic or oncogenic. However, during the past few years, some studies have shown that one ZFP may play both oncogenic and tumor-suppressive roles in HCC (Fig. 3). ZNF191, also known as ZNF24, belongs to the scan domain family’s transcription factors containing Krüppel-like zinc finger. It includes four zinc finger motifs that can encode potential DNA binding regions [148]. As a transcription factor that recognizes the TCAT motif explicitly, it plays an essential role in mammalian development, especially during the embryonic period. Within other areas of physiological function, it has been reported to promote the migration of endothelial cells and vascular smooth muscle cells and even promote DNA replication [148,149,150]. In HCC, ZNF191 was initially reported to enhance the transcriptional activity of β-catenin by binding to nucleotides located at − 1254/− 1224 on its promoter region, thereby upregulated the expression of its downstream target gene cyclinD1 and ultimately promoting the proliferation of HCC cells [151]. A few years later, the same team’s research about ZNF191 in HCC emerged, and this time it was playing an adverse role. Wu et al. revealed that ZNF191 activated DLG1 expression by directly binding to the DLG1 promoter through its typical TCAT repeat sequence, subsequently inhibiting the migration and YAP activation of HCC cells and ultimately the metastasis of HCC [152]. Recently, another study revealed that ZNF191 could activate the WNT signaling pathway through transcriptional upregulation of Wnt8B, which ultimately promoted the proliferation of HCC. Like β-catenin, the promoter region of Wnt8B was bound by ZNF191, which in turn enhanced Wnt8B transcriptional activity, thereby activating the Wnt signaling pathway [153]. It was worth noting that the two mechanisms could co-exist in HCC, which exerted both promotion and inhibition functions. Consequently, the expression level of ZNF191 and associated disease background must be clarified before targeting therapy for ZNF191-associated HCC, and the coexistence of these two mechanisms in the context of ZNF191 also deserves further investigation. We believe that ZNF191 has the potential to be a prognostic factor for HCC and a key therapeutic target as research progresses.

Fig. 3
figure 3

ZNF191 plays an opposite role in different stages of hepatocellular carcinoma. At early stage, ZNF191 activated the expression of CTNNB1 and its downstream gene Cyclin D1 by binding to the promoter region of CTNNB1, ultimately promoted the proliferation of HCC. At late stage, ZNF191 activated its expression by binding to the promoter of DLG1, thereby inhibited the activation of YAP and the migration of HCC cells, eventually inhibited the metastasis of HCC

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Conclusion and perspectives

As one of the largest transcription factor families in the human genome, ZFPs play diverse roles in cell biological functions, such as cell differentiation, apoptosis, transcriptional regulation, cell metabolism, immune response. In the past decades, there have been more reports about the role of ZFP in cancer, especially in HCC. HCC is still one of the cancers with the highest mortality rate in the world with many unsolved problems for early diagnosis and postoperative treatment. In this review, we described the role and related mechanisms of ZFPs in the development of HCC.

Above all, zinc finger motifs have different types, including Cys2His2 (C2H2) like, Gag knuckle, Treble clef, Zinc ribbon, Zn2/Cys6, TAZ2 domain like, Zinc binding loops and Metallothionein. The C2H2 zinc finger motif is the largest of all zinc finger motifs. In the C2H2 motif ZFP, in addition to the zinc finger structure, also contains other common domains, such as the KRAB domain, the BTB domain, SCAN domain and SET domain. These domains could bind to DNA, RNA or proteins to function. Secondly, ZFPs were also reported in regulating post-transcriptional modification and protein-protein interaction. Finally, ZFPs affect the development of HCC in different processes, such as cell cycle, apoptosis and even metabolism. Among them, ZNF191 shows a distinct effect in the early and late stages of HCC. Some ZFPs have also been reported as prognostic factors in HCC. For example, the high expression of ZBTB20 and ZNF689 in HCC patients was strongly associated with poor clinical prognosis as well as high recurrence rates [154, 155]. In a latest study, Sun et al. revealed that zinc finger protein 2 gene (ZIC2) was able to predict the prognosis of HCC, and ZIC2 was positively correlated with immune infiltration cells in HCC patients [156]. Therefore, due to the different roles that ZFPs play in HCC, it is possible to invent inhibitors for a specific ZFP or to interfere with the expression of its target gene, and these approaches may provide new thinking about ZFPs in HCC and even in other cancers.

In a word, ZFPs play an important role in tumorigenesis of HCC, and more mechanisms need to be further studied. The targeted drugs of ZFPs in HCC need to be further explored.

Availability of data and materials

Not applicable.

Abbreviations

ZNF:

zinc finger

HCC:

hepatocellular carcinoma

HBV:

hepatitis B virus

HCV:

hepatitis C virus

ZFP:

zinc finger protein

TF:

transcription factor

C2H2:

cys2his2

BTB:

Broad-complex, Tramtrack, and Bric-a-brac

KRAB:

Krüppel-associated box

SCAN:

SRE-ZBP, CTfin51, AW-1 and Number 18 cDNA

POZ:

poxvirus and zinc finger

ZSCAN:

zinc finger and scan

KZFPs:

Krüppel-associated box domain-containing zinc finger proteins

TEs:

transposable elements

ES:

embryonic stem

Trx:

trithorax

SAM:

S-adenosyl-L-methionine

ZEB1:

zinc finger E-box binding homeobox 1

HDGF:

hepatoma-derived growth factor

EC:

endometrial carcinoma

SMRZ:

striated muscle ring zinc finger protein

HSPCs:

hematopoietic stem and progenitor cells

PGC-1β:

proliferator-activated receptor gamma coactivator-1β

HIF-1α:

hypoxia-inducible factor-1α

DNL:

de novo lipogenesis

FLD:

fatty liver disease

HPCs:

hematopoietic progenitor cells

SSC:

skeletal stem/stromal cell

GSCs:

glioma stem cells

Gfi1:

growth factor independent 1 transcriptional repressor

LPS:

lipopolysaccharide

TLR:

toll-like receptor

ZFYVE1:

zinc finger FYVE-type containing 1

TLR3:

toll-like receptor 3

IL-17R:

IL-17 receptor

ER:

endoplasmic reticulum

CDKs:

cyclin-dependent kinases

CKIs:

cyclin-dependent kinase inhibitors

CDC6:

cell division cycle 6

MDIG:

mineral dust-induced gene

DISC:

death-inducing signaling complex

FADD:

fas-associated protein with death domain

Bax:

bcl2 associated x

Bcl-2:

bcl2 apoptosis regulator

PCAF:

p300/cbp-associated factor

CSCs:

cancer stem cells

ZFX:

zinc finger protein x-linked

ESCs:

embryonic stem cells

EpCAM:

epithelial cell adhesion molecule

EMT:

epithelial to mesenchymal transition

PKM2:

pyruvate kinase m2

PKM1:

pyruvate kinase m1

TNFAIP3:

tumor necrosis factorα-induced protein 3

PFKL:

phosphofructokinase, liver type

PFK:

phosphofructokinase

IKK:

the IkB kinase complex

References

  1. Kim S, Shendure J. Mechanisms of interplay between transcription factors and the 3D genome. Mol Cell. 2019;76(2):306–19. https://doi.org/10.1016/j.molcel.2019.08.010.

    Article  CAS  PubMed  Google Scholar 

  2. Chen A, Koehler AN. Transcription factor inhibition: lessons learned and emerging targets. Trends Mol Med. 2020;26:508–18.

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Krishna SS, Majumdar I, Grishin NV. Structural classification of zinc fingers: survey and summary. Nucleic Acids Res. 2003;31:532–50.

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Jen J, Wang YC. Zinc finger proteins in cancer progression. J Biomed Sci. 2016;23:53.

    PubMed  PubMed Central  Google Scholar 

  5. Gong D, Feng PC, Ke XF, Kuang HL, Pan LL, Ye Q, et al. Silencing long non-coding RNA LINC01224 inhibits hepatocellular carcinoma progression via MicroRNA-330-5p-induced inhibition of CHEK1. Mol Ther Nucleic Acids. 2020;19:482–97.

    CAS  PubMed  Google Scholar 

  6. McGlynn KA, Petrick JL, El-Serag HB. Epidemiology of hepatocellular carcinoma. Hepatology. 2021;73(Suppl 1):4–13.

    CAS  PubMed  Google Scholar 

  7. Zhang T, Huang Y, Liu W, Meng W, Zhao H, Yang Q, et al. Overexpression of zinc finger protein 687 enhances tumorigenic capability and promotes recurrence of hepatocellular carcinoma. Oncogenesis. 2017;6(7):e363. https://doi.org/10.1038/oncsis.2017.63.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Chen J, Li H, Huang J, Zhang L, Chen X, Wang Q, et al. Down-regulation of Gli-1 inhibits hepatocellular carcinoma cell migration and invasion. Mol Cell Biochem. 2014;393:283–91.

    CAS  PubMed  Google Scholar 

  9. Gai X, Lu Z, Tu K, Liang Z, Zheng X, Müller R. Caveolin-1 is up-regulated by GLI1 and contributes to GLI1-driven EMT in hepatocellular carcinoma. PLoS One. 2014;9:e84551.

    PubMed  PubMed Central  Google Scholar 

  10. Li J, He Y, Cao Y, Yu Y, Chen X, Gao X, et al. Upregulation of twist is involved in Gli1 induced migration and invasion of hepatocarcinoma cells. Biol Chem. 2018;399:911–9.

    CAS  PubMed  Google Scholar 

  11. Yi PS, Shu Y, Bi WX, Zheng XB, Feng WJ, He LY, et al. Emerging role of zinc finger protein A20 as a suppressor of hepatocellular carcinoma. J Cell Physiol. 2019;234:21479–84.

    CAS  PubMed  Google Scholar 

  12. Dang S, Zhou J, Chen Y, Chen P, Ji M, Shi B, et al. Dynamic expression of ZNF382 and its tumor-suppressor role in hepatitis B virus-related hepatocellular carcinogenesis. Oncogene. 2019;38(24):4804–19. https://doi.org/10.1038/s41388-019-0759-9.

    Article  CAS  PubMed  Google Scholar 

  13. Guan C, He L, Chang Z, Gu X, Liang J, Liu R. ZNF774 is a potent suppressor of hepatocarcinogenesis through dampening the NOTCH2 signaling. Oncogene. 2020;39:1665–80.

    CAS  PubMed  Google Scholar 

  14. Yang N, Wang L, Chen T, Liu R, Liu Z, Zhang L. ZNF521 which is downregulated by miR-802 suppresses malignant progression of hepatocellular carcinoma through regulating Runx2 expression. J Cancer. 2020;11:5831–9.

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Han M, Liao Z, Liu F, Chen X, Zhang B. Modulation of the TGF-beta signaling pathway by long noncoding RNA in hepatocellular carcinoma. Biomark Res. 2020;8:70.

    PubMed  PubMed Central  Google Scholar 

  16. Perez-Torrado R, Yamada D, Defossez PA. Born to bind: the BTB protein-protein interaction domain. Bioessays. 2006;28(12):1194–202. https://doi.org/10.1002/bies.20500.

    Article  CAS  PubMed  Google Scholar 

  17. Bardwell VJ, Treisman R. The POZ domain: a conserved protein-protein interaction motif. Genes Dev. 1994;8:1664–77.

    CAS  PubMed  Google Scholar 

  18. Stogios PJ, Downs GS, Jauhal JJ, Nandra SK, Prive GG. Sequence and structural analysis of BTB domain proteins. Genome Biol. 2005;6:R82.

    PubMed  PubMed Central  Google Scholar 

  19. Ahmad KF, Melnick A, Lax S, Bouchard D, Liu J, Kiang CL, et al. Mechanism of SMRT corepressor recruitment by the BCL6 BTB domain. Mol Cell. 2003;12:1551–64.

    CAS  PubMed  Google Scholar 

  20. Kang MI, Kobayashi A, Wakabayashi N, Kim SG, Yamamoto M. Scaffolding of Keap1 to the actin cytoskeleton controls the function of Nrf2 as key regulator of cytoprotective phase 2 genes. Proc Natl Acad Sci U S A. 2004;101(7):2046–51. https://doi.org/10.1073/pnas.0308347100.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Kreusch A, Pfaffinger PJ, Stevens CF, Choe S. Crystal structure of the tetramerization domain of the shaker potassium channel. Nature. 1998;392(6679):945–8. https://doi.org/10.1038/31978.

    Article  CAS  PubMed  Google Scholar 

  22. Furukawa M, He YJ, Borchers C, Xiong Y. Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. Nat Cell Biol. 2003;5:1001–7.

    CAS  PubMed  Google Scholar 

  23. Geyer R, Wee S, Anderson S, Yates J, Wolf DA. BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. Mol Cell. 2003;12(3):783–90. https://doi.org/10.1016/S1097-2765(03)00341-1.

    Article  CAS  PubMed  Google Scholar 

  24. Filion GJ, Zhenilo S, Salozhin S, Yamada D, Prokhortchouk E, Defossez PA. A family of human zinc finger proteins that bind methylated DNA and repress transcription. Mol Cell Biol. 2006;26(1):169–81. https://doi.org/10.1128/MCB.26.1.169-181.2006.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Schumacher C, Wang H, Honer C, Ding W, Koehn J, Lawrence Q, et al. The SCAN domain mediates selective oligomerization. J Biol Chem. 2000;275(22):17173–9. https://doi.org/10.1074/jbc.M000119200.

    Article  CAS  PubMed  Google Scholar 

  26. Ahmad KF, Engel CK, Prive GG. Crystal structure of the BTB domain from PLZF. Proc Natl Acad Sci U S A. 1998;95:12123–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Li X, Peng H, Schultz DC, Lopez-Guisa JM, Rauscher FR, Marmorstein R. Structure-function studies of the BTB/POZ transcriptional repression domain from the promyelocytic leukemia zinc finger oncoprotein. Cancer Res. 1999;59:5275–82.

    CAS  PubMed  Google Scholar 

  28. Huang M, Chen Y, Han D, Lei Z, Chu X. Role of the zinc finger and SCAN domain-containing transcription factors in cancer. Am J Cancer Res. 2019;9(5):816–36.

    CAS  PubMed  PubMed Central  Google Scholar 

  29. Pengue G, Calabro V, Bartoli PC, Pagliuca A, Lania L. Repression of transcriptional activity at a distance by the evolutionarily conserved KRAB domain present in a subfamily of zinc finger proteins. Nucleic Acids Res. 1994;22:2908–14.

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Sander TL, Haas AL, Peterson MJ, Morris JF. Identification of a novel SCAN box-related protein that interacts with MZF1B. The leucine-rich SCAN box mediates hetero- and homoprotein associations. J Biol Chem. 2000;275(17):12857–67. https://doi.org/10.1074/jbc.275.17.12857.

    Article  CAS  PubMed  Google Scholar 

  31. Williams AJ, Blacklow SC, Collins T. The zinc finger-associated SCAN box is a conserved oligomerization domain. Mol Cell Biol. 1999;19(12):8526–35. https://doi.org/10.1128/MCB.19.12.8526.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Eguchi T, Prince T, Wegiel B, Calderwood SK. Role and regulation of myeloid zinc finger protein 1 in Cancer. J Cell Biochem. 2015;116:2146–54.

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Bonchuk A, Boyko K, Fedotova A, Nikolaeva A, Lushchekina S, Khrustaleva A, et al. Structural basis of diversity and homodimerization specificity of zinc-finger-associated domains in Drosophila. Nucleic Acids Res. 2021;49(4):2375–89. https://doi.org/10.1093/nar/gkab061.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Imbeault M, Helleboid PY, Trono D. KRAB zinc-finger proteins contribute to the evolution of gene regulatory networks. Nature. 2017;543:550–4.

    CAS  PubMed  Google Scholar 

  35. Iyengar S, Farnham PJ. KAP1 protein: an enigmatic master regulator of the genome. J Biol Chem. 2011;286:26267–76.

    CAS  PubMed  PubMed Central  Google Scholar 

  36. Mannini R, Rivieccio V, D'Auria S, Tanfani F, Ausili A, Facchiano A, et al. Structure/function of KRAB repression domains: structural properties of KRAB modules inferred from hydrodynamic, circular dichroism, and FTIR spectroscopic analyses. Proteins. 2006;62(3):604–16. https://doi.org/10.1002/prot.20792.

    Article  CAS  PubMed  Google Scholar 

  37. Vissing H, Meyer WK, Aagaard L, Tommerup N, Thiesen HJ. Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins. FEBS Lett. 1995;369:153–7.

    CAS  PubMed  Google Scholar 

  38. Iuchi S. Three classes of C2H2 zinc finger proteins. Cell Mol Life Sci. 2001;58(4):625–35. https://doi.org/10.1007/PL00000885.

    Article  CAS  PubMed  Google Scholar 

  39. Nakahashi H, Kieffer KK, Resch W, Vian L, Dose M, Stavreva D, et al. A genome-wide map of CTCF multivalency redefines the CTCF code. Cell Rep. 2013;3:1678–89.

    CAS  PubMed  PubMed Central  Google Scholar 

  40. Schmitges FW, Radovani E, Najafabadi HS, Barazandeh M, Campitelli LF, Yin Y, et al. Multiparameter functional diversity of human C2H2 zinc finger proteins. Genome Res. 2016;26(12):1742–52. https://doi.org/10.1101/gr.209643.116.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Najafabadi HS, Mnaimneh S, Schmitges FW, Garton M, Lam KN, Yang A, et al. C2H2 zinc finger proteins greatly expand the human regulatory lexicon. Nat Biotechnol. 2015;33:555–62.

    CAS  PubMed  Google Scholar 

  42. Honer C, Chen P, Toth MJ, Schumacher C. Identification of SCAN dimerization domains in four gene families. Biochim Biophys Acta. 2001;1517:441–8.

    CAS  PubMed  Google Scholar 

  43. Ecco G, Imbeault M, Trono D. KRAB zinc finger proteins. Development. 2017;144(15):2719–29. https://doi.org/10.1242/dev.132605.

    Article  CAS  PubMed  Google Scholar 

  44. Del Rizzo PA, Trievel RC. Substrate and product specificities of SET domain methyltransferases. Epigenetics-Us. 2011;6(9):1059–67. https://doi.org/10.4161/epi.6.9.16069.

    Article  CAS  Google Scholar 

  45. Herz H, Garruss A, Shilatifard A. SET for life: biochemical activities and biological functions of SET domain-containing proteins. Trends Biochem Sci. 2013;38(12):621–39. https://doi.org/10.1016/j.tibs.2013.09.004.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Jiao Z, Wang L, Du H, Wang Y, Wang W, Liu J, et al. Genome-wide study of C2H2 zinc finger gene family in Medicago truncatula. BMC Plant Biol. 2020;20(1):401. https://doi.org/10.1186/s12870-020-02619-6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. McCarty AS, Kleiger G, Eisenberg D, Smale ST. Selective dimerization of a C2H2 zinc finger subfamily. Mol Cell. 2003;11(2):459–70. https://doi.org/10.1016/S1097-2765(03)00043-1.

    Article  CAS  PubMed  Google Scholar 

  48. Persikov AV, Wetzel JL, Rowland EF, Oakes BL, Xu DJ, Singh M, et al. A systematic survey of the Cys2His2 zinc finger DNA-binding landscape. Nucleic Acids Res. 2015;43(3):1965–84. https://doi.org/10.1093/nar/gku1395.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  49. Hashimoto H, Wang D, Horton JR, Zhang X, Corces VG, Cheng X. Structural basis for the versatile and methylation-dependent binding of CTCF to DNA. Mol Cell. 2017;66:711–20.

    CAS  PubMed  PubMed Central  Google Scholar 

  50. Caramel J, Ligier M, Puisieux A. Pleiotropic roles for ZEB1 in cancer. Cancer Res. 2018;78:30–5.

    CAS  PubMed  Google Scholar 

  51. Zhang P, Sun Y, Ma L. ZEB1: at the crossroads of epithelial-mesenchymal transition, metastasis and therapy resistance. Cell Cycle. 2015;14(4):481–7. https://doi.org/10.1080/15384101.2015.1006048.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. Schroth GP, Cook GR, Bradbury EM, Gottesfeld JM. Transcription factor IIIA induced bending of the Xenopus somatic 5S gene promoter. Nature. 1989;340(6233):487–8. https://doi.org/10.1038/340487a0.

    Article  CAS  PubMed  Google Scholar 

  53. Schleif R. DNA binding by proteins. Science. 1988;241:1182–7.

    CAS  PubMed  Google Scholar 

  54. Moroy T, Khandanpour C. Role of GFI1 in epigenetic regulation of MDS and AML pathogenesis: mechanisms and therapeutic implications. Front Oncol. 2019;9:824.

    PubMed  PubMed Central  Google Scholar 

  55. Wolf G, Greenberg D, Macfarlan TS. Spotting the enemy within: targeted silencing of foreign DNA in mammalian genomes by the Kruppel-associated box zinc finger protein family. Mob DNA. 2015;6:17.

    PubMed  PubMed Central  Google Scholar 

  56. Xiao YY, Lin L, Li YH, Jiang HP, Zhu LT, Deng YR, et al. ZEB1 promotes invasion and metastasis of endometrial cancer by interacting with HDGF and inducing its transcription. Am J Cancer Res. 2019;9:2314–30.

    CAS  PubMed  PubMed Central  Google Scholar 

  57. Jen J, Liu CY, Chen YT, Wu LT, Shieh YC, Lai WW, et al. Oncogenic zinc finger protein ZNF322A promotes stem cell-like properties in lung cancer through transcriptional suppression of c-Myc expression. Cell Death Differ. 2019;26(7):1283–98. https://doi.org/10.1038/s41418-018-0204-6.

    Article  CAS  PubMed  Google Scholar 

  58. Orth B, Sander B, Moglich A, Diederichs K, Eilers M, Lorenz S. Identification of an atypical interaction site in the BTB domain of the MYC-interacting zinc-finger protein 1. Structure. 2021;29(11):1230–40.e5. https://doi.org/10.1016/j.str.2021.06.005.

    Article  CAS  PubMed  Google Scholar 

  59. Dai KS, Liew CC. A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. J Biol Chem. 2001;276(26):23992–9. https://doi.org/10.1074/jbc.M011208200.

    Article  CAS  PubMed  Google Scholar 

  60. Yi D, Dempersmier JM, Nguyen HP, Viscarra JA, Dinh J, Tabuchi C, et al. Zc3h10 acts as a transcription factor and is phosphorylated to activate the thermogenic program. Cell Rep. 2019;29:2621–33.

    CAS  PubMed  PubMed Central  Google Scholar 

  61. Makita S, Takatori H, Nakajima H. Post-transcriptional regulation of immune responses and inflammatory diseases by RNA-binding ZFP36 family proteins. Front Immunol. 2021;12:711633. https://doi.org/10.3389/fimmu.2021.711633.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  62. Kidoya H, Muramatsu F, Shimamura T, Jia W, Satoh T, Hayashi Y, et al. Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis. Nat Commun. 2019;10(1):1072. https://doi.org/10.1038/s41467-019-09028-w.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  63. Vander HM, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324:1029–33.

    Google Scholar 

  64. Li Y, Kasim V, Yan X, Li L, Meliala I, Huang C, et al. Yin Yang 1 facilitates hepatocellular carcinoma cell lipid metabolism and tumor progression by inhibiting PGC-1beta-induced fatty acid oxidation. Theranostics. 2019;9(25):7599–615. https://doi.org/10.7150/thno.34931.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  65. Huang LT, Li X, Zhang L, Sun L, He X, et al. HIF-1-mediated suppression of acyl-CoA dehydrogenases and fatty acid oxidation is critical for cancer progression. Cell Rep. 2014;8:1930–42.

    CAS  PubMed  Google Scholar 

  66. Wu S, Kasim V, Kano MR, Tanaka S, Ohba S, Miura Y, et al. Transcription factor YY1 contributes to tumor growth by stabilizing hypoxia factor HIF-1alpha in a p53-independent manner. Cancer Res. 2013;73:1787–99.

    CAS  PubMed  Google Scholar 

  67. Liu G, Zhou L, Zhang H, Chen R, Zhang Y, Li L, et al. Regulation of hepatic lipogenesis by the zinc finger protein Zbtb20. Nat Commun. 2017;8(1):14824. https://doi.org/10.1038/ncomms14824.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  68. Chen T, You Y, Jiang H, Wang ZZ. Epithelial-mesenchymal transition (EMT): a biological process in the development, stem cell differentiation, and tumorigenesis. J Cell Physiol. 2017;232:3261–72.

    CAS  PubMed  PubMed Central  Google Scholar 

  69. Mani SA, Guo W, Liao MJ, Eaton EN, Ayyanan A, Zhou AY, et al. The epithelial-mesenchymal transition generates cells with properties of stem cells. Cell. 2008;133:704–15.

    CAS  PubMed  PubMed Central  Google Scholar 

  70. Goossens S, Janzen V, Bartunkova S, Yokomizo T, Drogat B, Crisan M, et al. The EMT regulator Zeb2/Sip1 is essential for murine embryonic hematopoietic stem/progenitor cell differentiation and mobilization. Blood. 2011;117:5620–30.

    CAS  PubMed  Google Scholar 

  71. Seuntjens E, Nityanandam A, Miquelajauregui A, Debruyn J, Stryjewska A, Goebbels S, et al. Sip1 regulates sequential fate decisions by feedback signaling from postmitotic neurons to progenitors. Nat Neurosci. 2009;12(11):1373–80. https://doi.org/10.1038/nn.2409.

    Article  CAS  PubMed  Google Scholar 

  72. Fardi M, Alivand M, Baradaran B, Farshdousti HM, Solali S. The crucial role of ZEB2: from development to epithelial-to-mesenchymal transition and cancer complexity. J Cell Physiol. 2019;234(9):14783–99. https://doi.org/10.1002/jcp.28277.

    Article  CAS  Google Scholar 

  73. Comijn J, Berx G, Vermassen P, Verschueren K, van Grunsven L, Bruyneel E, et al. The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. Mol Cell. 2001;7:1267–78.

    CAS  PubMed  Google Scholar 

  74. Takakura N, Watanabe T, Suenobu S, Yamada Y, Noda T, Ito Y, et al. A role for hematopoietic stem cells in promoting angiogenesis. Cell. 2000;102(2):199–209. https://doi.org/10.1016/S0092-8674(00)00025-8.

    Article  CAS  PubMed  Google Scholar 

  75. Tang Y, Feinberg T, Keller ET, Li X, Weiss SJ. Snail/slug binding interactions with YAP/TAZ control skeletal stem cell self-renewal and differentiation. Nat Cell Biol. 2016;18:917–29.

    CAS  PubMed  PubMed Central  Google Scholar 

  76. Caja L, Tzavlaki K, Dadras MS, Tan E, Hatem G, Maturi NP, et al. Snail regulates BMP and TGFβ pathways to control the differentiation status of glioma-initiating cells. Oncogene. 2018;37(19):2515–31. https://doi.org/10.1038/s41388-018-0136-0.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  77. Carpenter S, Ricci EP, Mercier BC, Moore MJ, Fitzgerald KA. Post-transcriptional regulation of gene expression in innate immunity. Nat Rev Immunol. 2014;14:361–76.

    CAS  PubMed  Google Scholar 

  78. Medzhitov R, Horng T. Transcriptional control of the inflammatory response. Nat Rev Immunol. 2009;9(10):692–703. https://doi.org/10.1038/nri2634.

    Article  CAS  PubMed  Google Scholar 

  79. Jin J, Zeng H, Schmid KW, Toetsch M, Uhlig S, Moroy T. The zinc finger protein Gfi1 acts upstream of TNF to attenuate endotoxin-mediated inflammatory responses in the lung. Eur J Immunol. 2006;36(2):421–30. https://doi.org/10.1002/eji.200535155.

    Article  CAS  PubMed  Google Scholar 

  80. Kawai T, Akira S. TLR signaling. Semin Immunol. 2007;19(1):24–32. https://doi.org/10.1016/j.smim.2006.12.004.

    Article  CAS  PubMed  Google Scholar 

  81. Zhong X, Feng L, Xu WH, Wu X, Ding YD, Zhou Y, et al. The zinc-finger protein ZFYVE1 modulates TLR3-mediated signaling by facilitating TLR3 ligand binding. Cell Mol Immunol. 2020;17(7):741–52. https://doi.org/10.1038/s41423-019-0265-6.

    Article  CAS  PubMed  Google Scholar 

  82. Fu M, Blackshear PJ. RNA-binding proteins in immune regulation: a focus on CCCH zinc finger proteins. Nat Rev Immunol. 2017;17:130–43.

    CAS  PubMed  Google Scholar 

  83. Maeda K, Akira S. Regulation of mRNA stability by CCCH-type zinc-finger proteins in immune cells. Int Immunol. 2017;29(4):149–55. https://doi.org/10.1093/intimm/dxx015.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  84. Carballo E, Lai WS, Blackshear PJ. Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin. Science. 1998;281:1001–5.

    CAS  PubMed  Google Scholar 

  85. Vinuesa CG, Cook MC, Angelucci C, Athanasopoulos V, Rui L, Hill KM, et al. A RING-type ubiquitin ligase family member required to repress follicular helper T cells and autoimmunity. Nature. 2005;435:452–8.

    CAS  PubMed  Google Scholar 

  86. Liang J, Wang J, Azfer A, Song W, Tromp G, Kolattukudy PE, et al. A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages. J Biol Chem. 2008;283:6337–46.

    CAS  PubMed  Google Scholar 

  87. Matsushita K, Takeuchi O, Standley DM, Kumagai Y, Kawagoe T, Miyake T, et al. Zc3h12a is an RNase essential for controlling immune responses by regulating mRNA decay. Nature. 2009;458(7242):1185–90. https://doi.org/10.1038/nature07924.

    Article  CAS  PubMed  Google Scholar 

  88. Tanaka H, Arima Y, Kamimura D, Tanaka Y, Takahashi N, Uehata T, et al. Phosphorylation-dependent Regnase-1 release from endoplasmic reticulum is critical in IL-17 response. J Exp Med. 2019;216(6):1431–49. https://doi.org/10.1084/jem.20181078.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  89. Hall TM. Multiple modes of RNA recognition by zinc finger proteins. Curr Opin Struct Biol. 2005;15(3):367–73. https://doi.org/10.1016/j.sbi.2005.04.004.

    Article  CAS  PubMed  Google Scholar 

  90. Zhang J, Wen X, Liu N, Li Y, Tang X, Wang Y, et al. Epigenetic mediated zinc finger protein 671 downregulation promotes cell proliferation and tumorigenicity in nasopharyngeal carcinoma by inhibiting cell cycle arrest. J Exp Clin Cancer Res. 2017;36:147.

    PubMed  PubMed Central  Google Scholar 

  91. Sun R, Xiang T, Tang J, Peng W, Luo J, Li L, et al. 19q13 KRAB zinc-finger protein ZNF471 activates MAPK10/JNK3 signaling but is frequently silenced by promoter CpG methylation in esophageal cancer. Theranostics. 2020;10(5):2243–59. https://doi.org/10.7150/thno.35861.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  92. Cao L, Wang S, Zhang Y, Wong K, Nakatsu G, Wang X, et al. Zinc-finger protein 471 suppresses gastric cancer through transcriptionally repressing downstream oncogenic PLS3 and TFAP2A. Oncogene. 2018;37:3601–16.

    CAS  PubMed  PubMed Central  Google Scholar 

  93. Huang C, Wu S, Li W, Herkilini A, Miyagishi M, Zhao H, et al. Zinc-finger protein p52-ZER6 accelerates colorectal cancer cell proliferation and tumour progression through promoting p53 ubiquitination. Ebiomedicine. 2019;48:248–63.

    CAS  PubMed  PubMed Central  Google Scholar 

  94. Li L, Liu X, He L, Yang J, Pei F, Li W, et al. ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin. Nat Commun. 2017;8:691.

    PubMed  PubMed Central  Google Scholar 

  95. Li Y, Yang Q, Guan H, Shi B, Ji M, Hou P. ZNF677 suppresses Akt phosphorylation and tumorigenesis in thyroid Cancer. Cancer Res. 2018;78(18):5216–28. https://doi.org/10.1158/0008-5472.CAN-18-0003.

    Article  CAS  PubMed  Google Scholar 

  96. Narita S, So A, Ettinger S, Hayashi N, Muramaki M, Fazli L, et al. GLI2 knockdown using an antisense oligonucleotide induces apoptosis and Chemosensitizes cells to paclitaxel in androgen-independent prostate Cancer. Clin Cancer Res. 2008;14(18):5769–77. https://doi.org/10.1158/1078-0432.CCR-07-4282.

    Article  CAS  PubMed  Google Scholar 

  97. Kudo K, Gavin E, Das S, Amable L, Shevde LA, Reed E. Inhibition of Gli1 results in altered c-Jun activation, inhibition of cisplatin-induced upregulation of ERCC1, XPD and XRCC1, and inhibition of platinum-DNA adduct repair. Oncogene. 2012;31(44):4718–24. https://doi.org/10.1038/onc.2011.610.

    Article  CAS  PubMed  Google Scholar 

  98. Chen H, Hu L, Luo Z, Zhang J, Zhang C, Qiu B, et al. A20 suppresses hepatocellular carcinoma proliferation and metastasis through inhibition of Twist1 expression. Mol Cancer. 2015;14(1):186. https://doi.org/10.1186/s12943-015-0454-6.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  99. Liao L, Chen C, Li N, Lin L, Huang B, Chang Y, et al. Y-box binding Protein-1 promotes epithelial-mesenchymal transition in Sorafenib-resistant hepatocellular carcinoma cells. Int J Mol Sci. 2021;22:224.

    CAS  Google Scholar 

  100. Ng ZL, Siew J, Li J, Ji G, Huang M, Liao X, et al. PATZ1 (MAZR) co-occupies genomic sites with p53 and inhibits liver Cancer cell proliferation via regulating p27. Front Cell Dev Biol. 2021;9:586150.

    PubMed  PubMed Central  Google Scholar 

  101. Li Z, Lu X, Liu Y, Zhao J, Ma S, Yin H, et al. Gain of LINC00624 enhances liver Cancer progression by disrupting the histone deacetylase 6/tripartite motif containing 28/zinc finger protein 354C corepressor complex. Hepatology. 2021;73(5):1764–82. https://doi.org/10.1002/hep.31530.

    Article  CAS  PubMed  Google Scholar 

  102. Coulombe P, Nassar J, Peiffer I, Stanojcic S, Sterkers Y, Delamarre A, et al. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nat Commun. 2019;10:2426.

    PubMed  PubMed Central  Google Scholar 

  103. Liang Y, Li Q, Chen K, Ni W, Zhan Z, Ye F, et al. Zinc finger protein 307 functions as a tumor-suppressor and inhibits cell proliferation by inducing apoptosis in hepatocellular carcinoma. Oncol Rep. 2017;38(4):2229–36. https://doi.org/10.3892/or.2017.5868.

    Article  CAS  PubMed  Google Scholar 

  104. Giannelli G, Koudelkova P, Dituri F, Mikulits W. Role of epithelial to mesenchymal transition in hepatocellular carcinoma. J Hepatol. 2016;65:798–808.

    CAS  PubMed  Google Scholar 

  105. Peukert K, Staller P, Schneider A, Carmichael G, Hänel F, Eilers M. An alternative pathway for gene regulation by Myc. EMBO J. 1997;16(18):5672–86. https://doi.org/10.1093/emboj/16.18.5672.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  106. Wang H, Xu H, Ma F, Zhan M, Yang X, Hua S, et al. Zinc finger protein 703 induces EMT and sorafenib resistance in hepatocellular carcinoma by transactivating CLDN4 expression. Cell Death Dis. 2020;11(4):225. https://doi.org/10.1038/s41419-020-2422-3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  107. Batlle E, Clevers H. Cancer stem cells revisited. Nat Med. 2017;23:1124–34.

    CAS  PubMed  Google Scholar 

  108. Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC. Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature. 2008;452:181–6.

    CAS  PubMed  Google Scholar 

  109. Giannelli G, Villa E, Lahn M. Transforming growth factor-β as a therapeutic target in hepatocellular carcinoma. Cancer Res. 2014;74:1890–4.

    CAS  PubMed  Google Scholar 

  110. He L, Fan X, Li Y, Chen M, Cui B, Chen G, et al. Overexpression of zinc finger protein 384 (ZNF 384), a poor prognostic predictor, promotes cell growth by upregulating the expression of cyclin D1 in hepatocellular carcinoma. Cell Death Dis. 2019;10(6):444. https://doi.org/10.1038/s41419-019-1681-3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  111. Karin M. NF- B as a critical link between inflammation and cancer. Cold Spring Harbor Perspect Biol. 2009;1:a000141.

    Google Scholar 

  112. Shahi P, Wang C, Lawson DA, Slorach EM, Lu A, Yu Y, et al. ZNF50 3/Zpo2 drives aggressive breast cancer progression by down-regulation of GATA3 expression. Proc Natl Acad Sci. 2017;114:3169–74.

    CAS  PubMed  PubMed Central  Google Scholar 

  113. Priem D, van Loo G, Bertrand MJM. A20 and cell death-driven inflammation. Trends Immunol. 2020;41(5):421–35. https://doi.org/10.1016/j.it.2020.03.001.

    Article  CAS  PubMed  Google Scholar 

  114. Lim S, Kaldis P. Cdks, cyclins and CKIs: roles beyond cell cycle regulation. Development. 2013;140:3079–93.

    CAS  PubMed  Google Scholar 

  115. Zhang L, Huo Q, Ge C, Zhao F, Zhou Q, Chen X, et al. ZNF143-mediated H3K9 Trimethylation upregulates CDC6 by activating MDIG in hepatocellular carcinoma. Cancer Res. 2020;80:2599–611.

    CAS  PubMed  Google Scholar 

  116. Tchakarska G, Sola B. The double dealing of cyclin D1. Cell Cycle. 2020;19(2):163–78. https://doi.org/10.1080/15384101.2019.1706903.

    Article  CAS  PubMed  Google Scholar 

  117. Peter ME. Apoptosis meets necrosis. Nature (London). 2011;471(7338):310–2. https://doi.org/10.1038/471310a.

    Article  CAS  Google Scholar 

  118. Sessler T, Healy S, Samali A, Szegezdi E. Structural determinants of DISC function: new insights into death receptor-mediated apoptosis signalling. Pharmacol Ther. 2013;140:186–99.

    CAS  PubMed  Google Scholar 

  119. Zheng X, Rumie Vittar NB, Gai X, Fernandez-Barrena MG, Moser CD, Hu C, et al. The transcription factor GLI1 mediates TGFb1 driven EMT in hepatocellular carcinoma via a SNAI1-dependent mechanism. PLoS One. 2012;7(11):e49581.

    CAS  PubMed  PubMed Central  Google Scholar 

  120. Gai X, Tu K, Li C, Lu Z, Roberts LR, Zheng X. Histone acetyltransferase PCAF accelerates apoptosis by repressing a GLI1/BCL2/BAX axis in hepatocellular carcinoma. Cell Death Dis. 2015;6:e1712.

    CAS  PubMed  PubMed Central  Google Scholar 

  121. Cui J, Liu J, Fan L, Zhu Y, Zhou B, Wang Y, et al. A zinc finger family protein, ZNF263, promotes hepatocellular carcinoma resistance to apoptosis via activation of ER stress-dependent autophagy. Transl Oncol. 2020;13:100851.

    PubMed  PubMed Central  Google Scholar 

  122. Beck B, Blanpain C. Unravelling cancer stem cell potential. Nat Rev Cancer. 2013;13:727–38.

    CAS  PubMed  Google Scholar 

  123. Plaks V, Kong N, Werb Z. The cancer stem cell niche: how essential is the niche in regulating stemness of tumor cells. Cell Stem Cell. 2015;16(3):225–38. https://doi.org/10.1016/j.stem.2015.02.015.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  124. Rhie SK, Yao L, Luo Z, Witt H, Schreiner S, Guo Y, et al. ZFX acts as a transcriptional activator in multiple types of human tumors by binding downstream from transcription start sites at the majority of CpG island promoters. Genome Res. 2018;28:310–20.

    CAS  PubMed Central  Google Scholar 

  125. Ni W, Perez AA, Schreiner S, Nicolet CM, Farnham PJ. Characterization of the ZFX family of transcription factors that bind downstream of the start site of CpG island promoters. Nucleic Acids Res. 2020;48(11):5986–6000. https://doi.org/10.1093/nar/gkaa384.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  126. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324(5928):797–801. https://doi.org/10.1126/science.1172482.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  127. Lai KP, Chen J, He M, Ching AKK, Lau C, Lai PBS, et al. Overexpression of ZFX confers self-renewal and chemoresistance properties in hepatocellular carcinoma. Int J Cancer. 2014;135(8):1790–9. https://doi.org/10.1002/ijc.28819.

    Article  CAS  PubMed  Google Scholar 

  128. Wang C, Fu SY, Wang MD, Yu WB, Cui QS, Wang HR, et al. Zinc finger protein X-linked promotes expansion of EpCAM+ cancer stem-like cells in hepatocellular carcinoma. Mol Oncol. 2017;11:455–69.

    CAS  PubMed  PubMed Central  Google Scholar 

  129. Bahrami A, Majeed M, Sahebkar A. Curcumin: a potent agent to reverse epithelial-to-mesenchymal transition. Cell Oncol. 2019;42(4):405–21. https://doi.org/10.1007/s13402-019-00442-2.

    Article  CAS  Google Scholar 

  130. Li LY, Yang JF, Rong F, Luo ZP, Hu S, Fang H, et al. ZEB1 serves an oncogenic role in the tumourigenesis of HCC by promoting cell proliferation, migration, and inhibiting apoptosis via Wnt/beta-catenin signaling pathway. Acta Pharmacol Sin. 2021;42(10):1676–89. https://doi.org/10.1038/s41401-020-00575-3.

    Article  CAS  PubMed  Google Scholar 

  131. Jiao W, Miyazaki K, Kitajima Y. Inverse correlation between E-cadherin and snail expression in hepatocellular carcinoma cell lines in vitro and in vivo. Br J Cancer. 2002;86(1):98–101. https://doi.org/10.1038/sj.bjc.6600017.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  132. Winkler M, Staniczek T, Kürschner SW, Schmid CD, Schönhaber H, Cordero J, et al. Endothelial GATA4 controls liver fibrosis and regeneration by preventing a pathogenic switch in angiocrine signaling. J Hepatol. 2021;74(2):380–93. https://doi.org/10.1016/j.jhep.2020.08.033.

    Article  CAS  PubMed  Google Scholar 

  133. Xiang Q, Zhou D, He X, Fan J, Tang J, Qiu Z, et al. The zinc finger protein GATA4 induces mesenchymal-to-epithelial transition and cellular senescence through the nuclear factor-κB pathway in hepatocellular carcinoma. J Gastroenterol Hepatol. 2019;34(12):2196–205. https://doi.org/10.1111/jgh.14684.

    Article  CAS  PubMed  Google Scholar 

  134. Shahi P, Slorach EM, Wang C, Chou J, Lu A, Ruderisch A, et al. The transcriptional repressor ZNF503/Zeppo2 promotes mammary epithelial cell proliferation and enhances cell invasion. J Biol Chem. 2015;290:3803–13.

    CAS  PubMed  Google Scholar 

  135. Yin G, Liu Z, Wang Y, Sun L, Wang L, Yao B, et al. ZNF503 accelerates aggressiveness of hepatocellular carcinoma cells by down-regulation of GATA3 expression and regulated by microRNA-495. Am J Transl Res. 2019;11:3426–37.

    CAS  PubMed  PubMed Central  Google Scholar 

  136. Faubert B, Solmonson A, DeBerardinis RJ. Metabolic reprogramming and cancer progression. Science. 2020;368(6487):w5473. https://doi.org/10.1126/science.aaw5473.

    Article  CAS  Google Scholar 

  137. Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science (Am Assoc Adv Sci). 2009;324:1029–33.

    CAS  Google Scholar 

  138. Jin X, Jin HR, Jung HS, Lee SJ, Lee J, Lee JJ. An atypical E3 ligase zinc finger protein 91 stabilizes and activates NF-κB-inducing kinase via Lys63-linked ubiquitination. J Biol Chem. 2010;285:30539–47.

    CAS  PubMed  PubMed Central  Google Scholar 

  139. Chen D, Wang Y, Lu R, Jiang X, Chen X, Meng N, et al. E3 ligase ZFP91 inhibits hepatocellular carcinoma metabolism reprogramming by regulating PKM splicing. Theranostics. 2020;10:8558–72.

    PubMed  PubMed Central  Google Scholar 

  140. Sun L, Suo C, Li S, Zhang H, Gao P. Metabolic reprogramming for cancer cells and their microenvironment: beyond the Warburg effect. Biochim Biophys Acta (BBA) - Rev Cancer. 2018;1870:51–66.

    CAS  Google Scholar 

  141. Ma A, Malynn BA. A20: linking a complex regulator of ubiquitylation to immunity and human disease. Nat Rev Immunol. 2012;12:774–85.

    CAS  PubMed  PubMed Central  Google Scholar 

  142. Catrysse L, Vereecke L, Beyaert R, van Loo G. A20 in inflammation and autoimmunity. Trends Immunol. 2014;35:22–31.

    CAS  PubMed  Google Scholar 

  143. Feng Y, Zhang Y, Cai Y, Liu R, Lu M, Li T, et al. A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma. Cell Death Dis. 2020;11.

  144. Lee J, Liu R, Li J, Zhang C, Wang Y, Cai Q, et al. Stabilization of phosphofructokinase 1 platelet isoform by AKT promotes tumorigenesis. Nat Commun. 2017;8(1):949. https://doi.org/10.1038/s41467-017-00906-9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  145. Kohnhorst CL, Kyoung M, Jeon M, Schmitt DL, Kennedy EL, Ramirez J, et al. Identification of a multienzyme complex for glucose metabolism in living cells. J Biol Chem. 2017;292(22):9191–203. https://doi.org/10.1074/jbc.M117.783050.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  146. Ruf B, Heinrich B, Greten TF. Immunobiology and immunotherapy of HCC: spotlight on innate and innate-like immune cells. Cell Mol Immunol. 2021;18(1):112–27. https://doi.org/10.1038/s41423-020-00572-w.

    Article  CAS  PubMed  Google Scholar 

  147. Zhang W, Zhangyuan G, Wang F, Jin K, Shen H, Zhang L, et al. The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation. Immunity. 2021;54:1168–85.

    CAS  PubMed  Google Scholar 

  148. Rousseau-Merck MF, Huebner K, Berger R, Thiesen HJ. Chromosomal localization of two human zinc finger protein genes, ZNF24 (KOX17) and ZNF29 (KOX26), to 18q12 and 17p13-p12, respectively. Genomics. 1991;9(1):154–61. https://doi.org/10.1016/0888-7543(91)90233-5.

    Article  CAS  PubMed  Google Scholar 

  149. Harper J, Yan L, Loureiro RM, Wu I, Fang J, D'Amore PA, et al. Repression of vascular endothelial growth factor expression by the zinc finger transcription factor ZNF24. Cancer Res. 2007;67:8736–41.

    CAS  PubMed  Google Scholar 

  150. Li J, Chen X, Gong X, Liu Y, Feng H, Qiu L, et al. A transcript profiling approach reveals the zinc finger transcription factor ZNF191 is a pleiotropic factor. BMC Genomics. 2009;10:241.

    CAS  PubMed  PubMed Central  Google Scholar 

  151. Liu G, Jiang S, Wang C, Jiang W, Liu Z, Liu C, et al. Zinc finger transcription factor 191, directly binding to beta-catenin promoter, promotes cell proliferation of hepatocellular carcinoma. Hepatology. 2012;55(6):1830–9. https://doi.org/10.1002/hep.25564.

    Article  CAS  PubMed  Google Scholar 

  152. Wu D, Liu G, Liu Y, Saiyin H, Wang C, Wei Z, et al. Zinc finger protein 191 inhibits hepatocellular carcinoma metastasis through discs large 1-mediated yes-associated protein inactivation. Hepatology. 2016;64:1148–62.

    CAS  PubMed  Google Scholar 

  153. Liu Y, Wu D, Cheng H, Chen L, Zhang W, Zou L, et al. Wnt8B, transcriptionally regulated by ZNF191, promotes cell proliferation of hepatocellular carcinoma via Wnt signaling. Cancer Sci. 2021;112(2):629–40. https://doi.org/10.1111/cas.14738.

    Article  CAS  PubMed  Google Scholar 

  154. Wang Q, Tan YX, Ren YB, Dong LW, Xie ZF, Tang L, et al. Zinc finger protein ZBTB20 expression is increased in hepatocellular carcinoma and associated with poor prognosis. BMC Cancer. 2011;11(1):271. https://doi.org/10.1186/1471-2407-11-271.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  155. Yi PS, Wu B, Deng DW, Zhang GN, Li JS. Positive expression of ZNF689 indicates poor prognosis of hepatocellular carcinoma. Oncol Lett. 2018;16:5122–30.

    PubMed  PubMed Central  Google Scholar 

  156. Sun L, Lin Y, Wang G, Zhang L, Hu L, Lu Z. Correlation of zinc finger protein 2, a prognostic biomarker, with immune infiltrates in liver cancer. Biosci Rep. 2021;41(1). https://doi.org/10.1042/BSR20203115.

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  1. Xinxin Li, Mengzhen Han and Hongwei Zhang contributed equally to this work.

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  1. Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China

    Xinxin Li, Mengzhen Han, Hongwei Zhang, Furong Liu, Yonglong Pan, Jinghan Zhu, Zhibin Liao, Xiaoping Chen & Bixiang Zhang

  2. Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases, Wuhan, 430030, China

    Xinxin Li, Mengzhen Han, Hongwei Zhang, Furong Liu, Yonglong Pan, Jinghan Zhu, Zhibin Liao, Xiaoping Chen & Bixiang Zhang

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Li, X., Han, M., Zhang, H. et al. Structures and biological functions of zinc finger proteins and their roles in hepatocellular carcinoma. Biomark Res 10, 2 (2022). https://doi.org/10.1186/s40364-021-00345-1

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ISSN: 2050-7771

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