- Open Access
Proprotein convertases in high-density lipoprotein metabolism
© Choi and Korstanje; licensee BioMed Central Ltd. 2013
- Received: 23 July 2013
- Accepted: 4 September 2013
- Published: 18 September 2013
The proprotein convertase subtilisin/kexins (PCSKs) are a serine endopeptidase family. PCSK members cleave amino acid residues and modulate the activity of precursor proteins. Evidence from patients and animal models carrying genetic alterations in PCSK members show that PCSK members are involved in various metabolic processes. These studies further revealed the molecular mechanism by which genetic alteration of some PCSK members impairs normal molecular and physiological functions, which in turn lead to cardiovascular disease. High-density lipoprotein (HDL) is anti-atherogenic as it removes excessive amount of cholesterol from blood and peripheral tissues. Several PCSK members are involved in HDL metabolism. PCSK3, PCSK5, and PCSK6 process two triglyceride lipase family members, endothelial lipase and lipoprotein lipase, which are important for HDL remodeling. Recent studies in our lab found evidence that PCSK1 and PCSK9 are also involved in HDL metabolism. A mouse model carrying an amino acid substitution in PCSK1 showed an increase in serum apolipoprotein A1 (APOA1) level. Another mouse model lacking PCSK9 showed a decrease in APOE-containing HDL. In this review, we summarize the role of the five PCSK members in lipid, glucose, and bile acid (BA) metabolism, each of which can influence HDL metabolism. We propose an integrative model in which PCSK members regulate HDL metabolism through various molecular mechanisms and metabolic processes and genetic variation in some PCSK members may affect the efficiency of reverse cholesterol transport. PCSK members are considered as attractive therapeutic targets. A greater understanding of the molecular and physiological functions of PCSK members will improve therapeutic strategies and drug efficacy for cardiovascular disease where PCSK members play critical role, with fewer adverse effects.
- Proprotein convertase subtilisin/kexin
- High-density lipoprotein cholesterol
- Reverse cholesterol transport
In the past two decades nine members of the highly conserved bacterial subtilisin- and yeast kexin-like serine protease family have been discovered and characterized. Analyses in animal models and in patients with mutations in PCSK members have revealed the molecular mechanism by which genetic alterations in PCSK members affect molecular and physiological functions in various metabolic diseases and endocrinological defects [1–4]. However, our understanding of how PCSK members are involved in HDL metabolism is still limited. The HDL biology has been updated in many reviews during the past decades [5–10], and we briefly summarize several key points: The major function of HDL is to remove excessive amounts of cholesterol from peripheral tissues via a mechanism called reverse cholesterol transport (RCT). HDL takes up cholesterol from low-density lipoprotein (LDL) or macrophage foam cells in atherosclerotic lesions and then delivers it to the liver for recycling or excretion. As a high level of HDL correlates with low risk of atherosclerotic cardiovascular diseases, HDL is considered an anti-atherogenic factor. Thus, abnormal regulation of HDL synthesis, remodeling and catabolism causes reduced HDL cholesterol concentration and HDL function, which then promotes the risk or progression of the disease.
Some PCSK members were previously found to be important in HDL metabolism. Data from our lab shows that PCSK1 is involved in regulating the levels of serum apolipoprotein A1 (APOA1), which is the major protein for HDL formation and remodeling (Choi et al., in preparation). PCSK3, PCSK5 and PCSK6 process two members in the triglyceride (TG) lipase family: endothelial lipases (EL) and lipoprotein lipases (LPL)  that play important role in regulating the HDL cholesterol concentration. We have also shown that PCSK9 influences HDL cholesterol concentration by regulating APOE-containing HDL levels .
Protein structure and principle of PCSK
Official and alternative protein name of the nine PCSK members
PCSKs and lipid metabolism
PCSK3, PCSK5, and PCSK6 are involved in lipid metabolism by modulating two members of the TG lipase family. The three PCSK members negatively influences EL activity by cleaving a carboxyl-terminal 18 kDa fragment in EL to suppress the enzymatic activity of EL (Figure 2A(2)) . EL cleavage was induced by transient expression of PCSK3, PCSK5, and PCSK6 in HEK293 cells . PCSK3 may also negatively influence EL activity through angiopoeitin-like protein 3 (ANGPTL3) (Figure 2A(3)). Adenoviral transduction of PCSK3 in hepatocytes led to less cleavage product and increased full-length ANGPTL3 . ANGPTL3 inhibited ~60% of EL activity by transfecting a plasmid expressing murine ANGPTL3 into HEK293 cells stably expressing EL . Lipoprotein lipase (LPL) is another member of the TG lipase family that is similarly inactivated by PCSK-mediated proteolytic cleavage (Figure 2A(4)) . Transient expression of PCSK3, PCSK5, and PCSK6 induced LPL cleavage in HEK293 cells .
The first work that linked PCSK9 with lipid metabolism was published by Abifadel and colleagues 10 years ago. Gain-of-function mutations in PCSK9 were found in familial autosomal dominant hypercholesterolemia patients . The mutations increase LDLR degradation and decrease LDL clearance, which results in an increase in circulating LDL. PCSK9 positively regulates APOB concentration (Figure 2(5)) and inhibition of PCSK9 leads to an increase of LDLR (Figure 2A(6)) and APOB-containing LDL particles are more rapidly cleared. PCSK9 also promotes degradation of the VLDL receptor (VLDLR) and APOE receptor type 2 (LRP8), both of which are important in TG and VLDL metabolism. PCSK9 negatively regulates VLDL concentration through the two receptors (Figure 2A(7)). Ectopic expression of the PCSK9 gene in HEK293 cells reduced the levels of LRP8 and VLDLR, while PCSK9 inhibition in CHO-1 and HEK293 cell lines increased the levels of VLDLR .
PCSKs and glucose metabolism
PCSK1 and PCSK9 are involved in glucose metabolism (Figure 2B) . PCSK1 and PCSK9 positively regulate pro-insulin conversion to insulin by proteolytic cleavage in the pancreas. A non-synonymous single nucleotide polymorphism in human PCSK1 (rs6232) substitutes asparagine with aspartic acid at codon 221 (N221D). Transfecting recombinant N221D PCSK1 protein in human embryonic kidney 293 cells reduces the proteolytic cleavage of its substrate . The rs6232 variant is associated with an increase in fasting blood glucose level and a decrease in pancreatic β-cell function and insulin resistance . Insulin was decreased in the pancreas of PCSK9 KO C57BL/6 males, and the males exhibited hyperinsulinemic, hyperglycemic, and glucose-intolerant phenotypes .
PCSKs and BA metabolism
PCSK1 and PCSK5 are involved in BA metabolism (Figure 2C) and positively regulate CCK in the small intestine by converting procholecystokinin (proCCK) to CCK . An immunohistochemical analysis and an in vitro cleavage assay showed that PCSK1 co-localizes with proCCK and positively regulates proCCK level. The uncleaved proCCK level was increased and the cleaved CCK level was decreased in the small intestine of mice lacking PCSK1 compared to wild-type control mice . Treating cultured mouse small intestinal cells with small-interfering (si) RNA against Pcsk5 significantly reduced the secretion of cleaved CCK into the cell culture medium . The secretion of CCK from the small intestine stimulates BA secretion from the gall bladder to the gut. BAs promote lipid absorption and modulate cholesterol levels through enterohepatic circulation. Defects in BA metabolism cause jaundice of the eye and skin.
PCSKs and HDL metabolism
Normal regulation of insulin, glucose, hormones, and lipids is important for HDL homeostasis, which involves synthesis, action, and elimination of HDL lipoproteins [35, 36]. Mutations in PCSK1 and PCSK5 may cause abnormal HDL metabolism through mis-regulation of signal molecules in BA. For example, CCK or chenodeoxycholic acid (CDCA) positively influences BA metabolism (Figure 3(5)). When dietary lipids and other nutrients arrive in the gut, cholecystokinin (CCK) secreted from the small intestine stimulates BA secretion from the gall bladder to the gut. The majority of the BA is re-absorbed into the liver via enterohepatic circulation and the reminder is excreted through feces. CDCA is a major component in the BA and functions as a signaling molecule in HDL metabolism. CDCA binds to the farnesoid X-activated receptor (FXR) that regulates lipid and cholesterol metabolism . FXR, a member of the nuclear hormone receptor superfamily, forms a heterodimer with the retinoid X receptor (RXR). The FXR/RXR heterodimer functions as a transcription factor that positively influences phospholipid transfer protein (PLTP) mRNA transcription (Figure 3(6)). Co-transfecting FXR and RXR expression plasmids and CDCA treatment in monkey kidney cells increased PLTP promoter activity. Cholic acid, another major component in the BA, is involved in PLTP regulation . Mice fed a chow diet with cholic acid exhibited increased hepatic PLTP mRNA levels. PLTP is an important factor for HDL conversion and positively influence HDL level (Figure 3(7)). Mice lacking the Pltp gene displayed reduced levels of HDL cholesterol (65%) and APOA1 protein (85%) (Figure 3(8)) compared to wild-type control mice . In humans, a non-synonymous mutation of L196W in the PLTP gene impairs HDL conversion [40, 41].
Maintenance of normal HDL metabolism is critical for normal physiological function of HDL. Reverse cholesterol transport (RCT) is an anti-atherogenic process that occurs between lipoproteins (Figure 3(9)) or between lipoproteins and macrophage foam cells (Figure 3(10)). The efficiency of RCT can be analyzed by measuring the amount of cholesterol in medium and cells. EL deficiency affects efficiency of cholesterol efflux from macrophages. EL knockdown using small hairpin (sh) RNA reduced APOA1-mediated cholesterol efflux . PCSK9 KO decreases the efficiency of cholesterol efflux . Treating PCSK9 KO mouse serum to cultured macrophage foam cells exhibits decreased cholesterol efflux. Thus, abnormal HDL regulation caused by genetic alterations in some PCSK members may interfere with reverse cholesterol transport efficiency.
Normal insulin processing, which influences glucose and triglyceride metabolism, is critical for maintaining normal HDL metabolism . Type II diabetic patients show decreased small HDL particles . Hypertriglyceridemia patients with hyperglycemic phenotype exhibited increased TG-rich HDL with lower stability and shorter plasma residence time compare to cholesterol-rich HDL (Figure 2(11)) . The TG-rich HDL is rapidly catabolized, which subsequently decreases plasma HDL cholesterol concentration . Another potential cause of abnormal HDL metabolism is glycation that leads to conformation changes of proteins. Glycation of amino-acid residues in APOA1 alters its binding affinity to ATP-binding cassette sub-family G member 1 (ABCG1) in the human acute monocytic leukemia cell lines (THP-1). As a result, the ability of cholesterol efflux from THP-1 cells was significantly reduced (< 70%) compared to unglycated APOA1 .
Some of the PCSK members have functional redundancy by processing common substrates: insulin by PCSK1 and PCSK9; CCK by PCSK1 and PCSK5; TG lipases (EL and LPL) by PCSK3, PCSK5, and PCSK6. In the latter case, all 3 PCSKs modulate the activity of the TG lipases, but the efficiency of the modulation varies: for EL inactivation PCSK6 has the highest efficiency while PCSK3 has the lowest efficiency; for LPL activation PCSK3 has the highest efficiency while PCSK5 has the lowest efficiency . The PCSK members also regulate one another. The activity of PCSK9 is negatively influenced by two other PCSK members: PCSK3 (Figure 3(12)) and PCSK5 (Figure 3(13)). PCSK3 cleaves PCSK9 at the Arg218-Gln219 peptide bond in HEK293 cells, and PCSK5 cleaves the same bond, but with lower efficiency compared to PCSK3 .
The biology and therapeutic targeting of PCSK members has been recently reviewed . Particularly, therapeutic inhibition of PCSK9 has been proven to be a promising pro-atherogenic LDL cholesterol-lowering treatment. Co-treatment of PCSK inhibitors with drugs that suppress cholesterol synthesis is even more effective in reducing LDL cholesterol in hypercholesterolemia patients . Also, PCSK9 inhibition in the patients did not cause negative impact on anti-atherognic HDL cholesterol concentration in early-phase clinical trial phase I or phase II [47–49]. However, the effect of PCSK9 inhibition on HDL cholesterol concentration is controversial. Some studies used mice showed that PCSK9 inhibition decreases HDL cholesterol concentration [12, 32]. Because HDL cholesterol regulation and metabolism differ between mouse and human, we speculate that in human, although the inhibition of PCSK9 affects LDLR level, it might not cause strong enough effect to see significant measurable impact on HDL cholesterol concentration. In humans, cholesterol ester transfer protein (CETP) is a critical factor for regulating HDL cholesterol concentration by transferring cholesterol from HDL to non-HDL particles. In mice lacking CETP, cholesterol is enriched in APOE-containing HDL particles and the particles are cleared through LDLR. In other words, the effect of an increase of LDLR level by PCSK9 inhibition on HDL cholesterol concentration in humans might be smaller relative to its effect in mice. At the same time, studies in nonhuman primates, which have CETP and have similar HDL cholesterol metabolism to humans in comparison to mice, show inconsistent results in regards to the effect of PCSK9 inhibition on HDL cholesterol concentration [34, 50]. Treatment with neutralizing antibody against PCSK9 for the first 7 days of treatment decreased HDL cholesterol concentration . Meanwhile, knockdown of PCSK9 by RNAi did not decrease HDL cholesterol concentration . The data from these studies suggest that there are likely to be other factors causing the inconsistent results between studies. First, it is possible that the inconsistent results between studies might be caused by the dosage of PCSK9 inhibition. Knockout of PCSK9 in mice [12, 32] decreased HDL cholesterol concentrations and knockdown of PCSK9 by RNAi  and overexpression of PCSK9 by adenovirus  did not decrease HDL cholesterol concentrations. Second, it is also possible that whether or not PCSK9 targeting directs liver might cause the inconsistent results. Liver-specific siRNA silencing  and overexpression of PCSK9 by adenovirus, which is known to primarily target the liver, do not decrease HDL cholesterol concentration. PCSK9 in other tissues remains unaffected and might contribute to the lack of decreased HDL cholesterol concentration. The no change in HDL cholesterol concentration despite the change in LDLR by PCSK9 inhibition  and overexpression  remains unclear and additional studies would be needed to address this observation. If the method is indeed the cause of the variation in HDL cholesterol, then additional studies may provide valuable information about the effectiveness of different methods. At the same time, the use of neutralizing antibodies against PCSK9 in several clinical trials [47–49] show no negative impact on HDL cholesterol concentration. Considering the importance of PCSK9 as a promising therapeutic target, it will be important to understand whether the inconsistent results are due to different dosage (knockout versus knockdown), CETP (presence vs absence), or target (gene vs protein; liver vs organism). The molecular and physiological functions of PCSK members in HDL metabolism are likely more complex than we currently know. In particular, our understanding of compensatory mechanisms between PCSK members is also limited. We look forward to continued studies that will improve our understanding and allow more precise pharmacological regulation of PCSK for the treatment of metabolic disease.
SC: Ph.D. candidate in the cooperative pre-doctoral program at The Jackson Laboratory, Bar Harbor, ME 40609, USA. RK: assistant professor at the Jackson Laboratory and adjunct research scientist at The Mount Desert Island Biological Laboratory, Bar Harbor, ME 04609, USA.
This work was supported by HL081162, HL077796, and HL095668 from the National Heart, Lung and Blood Institute. The authors thank Dr. Xiaosong Wang for critical review of the manuscript; Joanne Currer, Kyle Beauchemin, Elisabeth Adkins, Aleksandra Aljakna, and George Sutphin for manuscript preparation.
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