- Letter to the Editor
- Open Access
Chemerin is elevated in multiple myeloma patients and is expressed by stromal cells and pre-adipocytes
Biomarker Researchvolume 6, Article number: 21 (2018)
Chemerin is a recently discovered adipokine shown to be involved in both inflammatory and metabolic processes. Here, we demonstrate that chemerin serum levels are elevated in patients with multiple myeloma and that it increases with disease progression. We found that chemerin is expressed by stromal cells and preadipocytes, whereas its receptor CCRL2 is expressed by primary myeloma cells, suggesting a paracrine signaling loop between bone marrow stromal cells/adipocytes and myeloma cells. This is the first study exploring chemerin and its receptors in multiple myeloma.
To the editor,
Multiple myeloma is a hematological malignancy caused by clonal proliferation of malignant plasma cells in the bone marrow. Although novel therapies have increased survival of multiple myeloma patients, it remains an incurable disease. Myeloma cells depend on the microenvironment for adhesion, survival and drug resistance. Targeting the microenvironment is vital for novel therapy strategies .
Bone marrow adipocytes have emerged as important players in cancer development . They serve as energy reservoirs, secrete fatty acids and adipokines and take part in regulating bone homeostasis. Since myeloma is a disease of the elderly, and adiposity increases with age, the role of adipocytes in myeloma are currently gaining interest. Adiposity has already been shown to increase the risk of myeloma in several studies [3, 4]. Further, it has been shown that adipocytes may affect proliferation, apoptosis and migration of myeloma cells as well as protecting them during chemotherapy [5, 6].
Chemerin (also known as TIG2 and RARRES2) was initially identified as an adipokine involved in adipogenesis and adipocyte metabolism . Chemerin is a modulator of inflammation and it has been suggested as a tumor marker in several cancers .
Due to the emerging important role of adipocytes in myeloma, and the role of chemerin in cancers in particular, we measured chemerin serum levels in patients and age— and gender matched healthy volunteers. The patient serum samples were collected at diagnosis during a randomized phase 3 clinical trial, which compared the effect of two different doses of pamidronate on bone in the years 2001–2005 . Out of the original 504 patients, serum samples were available for 122 patients (patient characteristics, Table 1). We found a significant difference between chemerin concentration in patients (mean = 199.2 ng/ml ± 88.2, n = 122) and healthy controls (mean = 156.5 ng/ml ± 52.5, n = 58) (p < 0.001, Mann-Whitney) as shown in Fig. 1a. Chemerin serum levels were significantly higher in patients in ISS stage III (mean = 255.2, ± 106.5, n = 28) compared to patients in ISS stage I (mean = 168.2, ± 65.4, n = 33) (p < 0.001), and patients in stage II (mean = 189.5, ± 75.8, n = 47) (p < 0.05, Kruskal Wallis with Dunns post hoc test) as shown in Fig. 1b. As chemerin levels correlated with disease stage we compared overall survival in patients with above (> 192.1 ng/ml) and below median (≤ 192.1) serum chemerin levels. Overall survival did not differ significantly between the two groups (median survival 61.9 months (n = 61) for the low chemerin group versus median survival 47.8 months (n = 61) for the high chemerin group, p = 0.318 (Mantel-Cox Test). In this patient cohort, chemerin correlated with CRP (spearman r = 0.191 p < 0.05) and serum β-microglobulin (spearman r = 0.332, p < 0.001), but not with number of plasma cells (%), the M-component (g/dl), body mass index, s-LDH (ukat/L) or s-calcium (mmol/L). Chemerin serum levels did not associate with bone disease status, or time to SRE. To compare levels of chemerin in the blood and the bone marrow, we analyzed matching blood and bone marrow- plasma samples from 10 myeloma patients (8 from time of diagnosis and 2 from relapse) available at Biobank1®. Chemerin blood plasma levels correlate strongly with chemerin bone marrow plasma levels (spearman r = 0.894, p < 0.005) as shown in Fig. 1c. There was a tendency to increased amounts of chemerin in the bone marrow plasma compared with levels in blood plasma ((mean = 251.3 ng/ml vs 214.7 ng/ml), n = 10).
To examine which cells may be producers of chemerin in the bone marrow we examined gene expression by qPCR in stromal cells isolated from myeloma patients (n = 15), primary myeloma cells (n = 24) and myeloma cell lines (n = 9). We found that chemerin was significantly higher expressed in stromal cells compared with primary myeloma cells, and that myeloma cell lines expressed lower levels than primary myeloma cells, Fig. 1d. Since chemerin was initially identified as an adipokine we further measured chemerin protein levels in the supernatants from bone marrow-derived mesenchymal stromal cells (n = 3) differentiated towards adipocytes using the hMSC Adipogenic Differentiation BulletKit™ Medium (Lonza). Interestingly, pre-adipocytes produce and secrete chemerin at high levels, as shown in Fig. 1e. The increase in chemerin protein levels corresponded with an increase in chemerin mRNA levels (not shown).
Chemerin mediates effect through its receptors CMKLR1 and GPR1, but it can also bind to CCRL2, which is a non-signaling receptor . To examine receptor expression for chemerin in primary myeloma cells, we utilized the CoMMpass data series, which contains RNA-sequencing data from 673 newly diagnosed myeloma patients. We found that CCRL2 mRNA was expressed in most of the patients while CMKLR1 mRNA was expressed in a fraction of the patients. In line with qPCR results in Fig. 1c chemerin (RARRES2) was not expressed by primary myeloma cells, Fig. 1f. Interestingly, primary myeloma cells expressed more CCRL2 and CMKLR1 mRNA compared with multiple myeloma cell lines (Additional file 1: Figure S1).
In conclusion, chemerin levels in serum are elevated in patients with multiple myeloma compared to healthy controls. Chemerin serum levels correlate with disease stage. Stromal cells obtained from myeloma patients and pre-adipocytes produce chemerin, whereas the receptors CCRL2 and CMKLR1 are expressed by myeloma cells. Thus, we propose that chemerin may mediate a paracrine signaling loop between stromal cells/adipocytes and myeloma cells.
C-C chemokine receptor-like 2
Chemerin Chemokine-Like Receptor 1
G Protein-Coupled Receptor 1
Human mesenchymal stromal cell
International staging system
Retinoic Acid Receptor Responder 2
Skeletal related event
Gooding S, Edwards CM. New approaches to targeting the bone marrow microenvironment in multiple myeloma. Curr Opin Pharmacol. 2016;28:43–9.
Morris EV, Edwards CM. Bone marrow adipose tissue: a new player in Cancer metastasis to bone. Front Endocrinol. 2016;7:90.
Hofmann JN, et al. Body mass index and physical activity at different ages and risk of multiple myeloma in the NIH-AARP diet and health study. Am J Epidemiol. 2013;177(8):776–86.
Wallin A, Larsson SC. Body mass index and risk of multiple myeloma: a meta-analysis of prospective studies. Eur J Cancer. 2011;47(11):1606–15.
Caers J, et al. Neighboring adipocytes participate in the bone marrow microenvironment of multiple myeloma cells. Leukemia. 2007;21(7):1580–4.
Yu W, et al. Adipocytes secreted leptin is a pro-tumor factor for survival of multiple myeloma under chemotherapy. Oncotarget. 2016;7(52):86075–86.
Goralski KB, et al. Chemerin, a novel Adipokine that regulates Adipogenesis and adipocyte metabolism. J Biol Chem. 2007;282(38):28175–88.
Fatima SS, et al. New roles of the multidimensional adipokine: Chemerin. Peptides. 2014;62(Supplement C):15–20.
Gimsing P, et al. Effect of pamidronate 30 mg versus 90 mg on physical function in patients with newly diagnosed multiple myeloma (Nordic myeloma study group): a double-blind, randomised controlled trial. The Lancet Oncology. 2010;11(10):973–82.
Yoshimura T, Oppenheim JJ. Chemokine-like receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2); two multifunctional receptors with unusual properties. Exp Cell Res. 2011;317(5):674–84.
We thank Biobank1® for collecting and storing patient material.
This work was supported by funds from the Norwegian Cancer Society (#4500930), The Kristian Gerhard Jebsen Foundation (SKGJ-MED-007), the Norwegian Research Council (#223255 and #193072), the Liaison Committee for education, research and innovation in Central Norway (#9006100) and the Cancer Fund (Kreftfondet) at St Olavs Hospital, Trondheim.
Availability of data and materials
Data sharing is not applicable to this article as no datasets were generated during the current study.
Ethics approval and consent to participate
The samples were gathered during a randomized trail (NCT00376883) .
Some samples were available from Biobank1®. This study was approved by the Regional Ethics Committee (REK 2011–2029). All participants provided written informed consent.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Figure S1. Expression of CCRL2 and CMKLR1 in primary myeloma cells (pMM, n = 24) and cell lines (n = 9) analyzed by qPCR. GAPDH was used as an endogenous control. (DOCX 51 kb)