Fig. 8

Exogenous and intracellular IL15 differentially regulate IL-15-mediated signaling, cell morphological changes, and migration. A, A Transwell migration assay was performed on IL-15-overexpressing A549 and PC9 cells treated with exogenous IL-15 (100 ng/mL) for 24 h. Scale bar, 100 μm.*, p < 0.05; **, p < 0.01; ***, p < 0.001. B-D, IL-15-overexpressing A549 and PC9 cells were treated with rIL-15 for 24 h. Then, cell morphological changes were evaluated by confocal fluorescence microscopy (B).Scale bar, 10 μm.****, p < 0.0001. The expression levels of the indicated proteins were measured by immunoblotting (C). Cell lysates were subjected to a pulldown assay to detect GTP-RhoA and the indicated proteins (D)