Fig. 7

Exogenous IL15 inhibits stress fiber and focal adhesion formation via downregulation of RhoA activity. (A-E), A549 and PC9 cells were treated with rIL-15 (100 ng/mL). The formation of actin stress fibers (A) and vinculin expression (B) were assessed. Scale bar, 20 μm. ****, p < 0.0001. A pulldown assay was performed to detect the active form of RhoA (C). Phosphorylated MLC2 (D) and FAK (E) were analyzed by immunoblotting. (F), A549 and PC9 cells were treated with rIL-15 (100 ng/mL) for 6–24 h. The expression levels of the indicated proteins were measured by immunoblotting