Fig. 6

Exogenous IL15 inhibits tumor cell migration and invasion. (A and B), Transwell migration (A) and invasion (B) assays were performed on A549 and PC9 cells in the presence or absence of rIL15. The data are presented as the means ± SDs and were analyzed with one-way ANOVA. Scale bar, 100 μm. *, p < 0.05; **, p < 0.01; ****, p < 0.0001. (C), A549 cells were cultured with rIL15 (100 ng/mL). The motion trajectory of the A549 cells was recorded using a live-cell analysis system for 24 h. The distance and speed data are presented as the means ± SDs and were analyzed with two-tailed Student’s t test. ****, p < 0.0001. (D), A Transwell migration assay was performed on IL-15-overexpressing A549 and PC9 cells in the presence or absence of an anti-IL15 neutralizing antibody (10 µg/mL). Scale bar, 100 μm