Fig. 2
Cancer cell-intrinsic IL-15 promotes metastasis in vivo. (A and B), A549 cells transfected with shIL15 and shCtrl (A) or Lv-hIL-15 and Lv-hCtrl (B) were intravenously injected into NCG mice (1 × 106 cells/mouse, n = 3). The mice were euthanized on day 7 after i.v. injection. Lung tissues were subjected to H&E staining. The area of lung metastasis was calculated. Scale bar, 50 μm. ****, p < 0.0001. (C), Transwell migration and invasion assays were performed on LLC cells transduced with Lv-mIL-15 and Lv-mCtrl. Scale bar, 100 μm. ****, p < 0.0001. (D), LLC cells transduced with Lv-mIL-15 or Lv-mCtrl were injected into C57BL/6 mice (1 × 106 cells/mouse, n = 4) via the tail vein. The mice were euthanized on day 5 after i.v. injection. Lung tissues were subjected to H&E staining. Scale bar, 50 μm. ****, p < 0.0001. The area of lung metastasis was calculated as described in A and B. The data in the bar graphs are presented as the means ± SDs and were analyzed with a two-tailed Student’s t test