Fig. 1

Cancer cell-intrinsic IL-15 promotes cell migration, invasion, and motility. (A), IHC staining of IL15 in lung adenocarcinoma tissue and corresponding normal lung tissue (n = 6). Scale bar, 50 μm. **, p < 0.01. (B-E), Transwell migration (B and C) and invasion (D and E) assays were performed on A549 cells and PC9 cells transfected with siRNA-IL15 and siRNA-Ctrl (B, D) or with Lv-hIL-15 and Lv-hCtrl (C, E). Scale bar, 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. (F and G), A549 cells were transfected with siRNA-IL15 or siRNA-Ctrl for 48 h, and subsequently, the motion trajectory of the cells was recorded using a live-cell analysis system for 24 h (F). Alternatively, the motion trajectories of A549 cells transduced with Lv-hIL-15 or Lv-hCtrl were recorded for 24 h (G). The motion images were analyzed using manual tracking in ImageJ with the Chemotaxis and Migration Tool plugin. The data in the bar graphs are presented as the means ± SDs and were analyzed with two-tailed Student’s t test. ****, p < 0.0001