Fig. 6

Proteomic landscape analyses of molecular mechanisms associated with up-regulation of hMT3 in Huh7 cells. A Venn diagram showing exclusiveness and commonness of proteins identified in HuH7^mock and Huh7^hMT3 cells. B Numbers of proteins commonly expressed in Huh7^mock and Huh7^hMT3 cells and the distribution of their deregulation. Up-regulation, fold ratio ≥ 1.5; down-regulation, fold ratio ≤ 0.5. Classification of top up-regulated proteins (including exclusively expressed proteins in Huh7^hMT3) according to their (C) biological processes and (D) biological pathways. E Interactome showing relations of biological processes driven by down-regulated proteins in Huh7^hMT3vs. Huh7^mock, metabolism of lipids (orange nodes), metabolism of amino acids (blue nodes), metabolism of nucleobase-containing small molecules (red nodes), cellular response to stress (green nodes), positive regulation of lamellipodium (yellow nodes) and translation (purple nodes). F Top 5 clusters of enriched terms within the metabolism of lipids in Huh7^hMT3. G Representative micrographs of BODIPY-stained lipid bodies’ content in mouse primary hepatocytes and (H) their relative quantification (lipid bodies/cell ± SEM, n ≥ 12). ***p ≤ 0.005 ****p ≤ 0.001