Fig. 3

In Huh7 cells, hMT3 affects mitochondrial flux, oxidative stress, apoptosis, and cell cycle. Metabolic analysis of Huh7^mock and Huh7^hMT3 cells expressed as (A) OCR and (B) ECAR values (n = 6). C Sorafenib-mediated induction of total ROS, (D) mitochondrial superoxide and (E) SOD activity in Huh7^mock and Huh7^hMT3 cells. *p ≤ 0.05, **p ≤ 0.001, ***p ≤ 0.0005 (Student’s test, two-sided). F Scatter plots of double-stained (Annexin V-Dy647/7-AAD) cells showing markedly lower ability of sorafenib to trigger apoptosis in Huh7^hMT3 and BCLC-3^WT compared to Huh7^mock cells. G Quantification of flow cytometry analysis of apoptosis induction by sorafenib. Data are expressed as mean ± SEM. ***p ≤ 0.0005 (Student’s test, two-sided). H Cell cycle histograms of PI-stained cells cultured with or without sorafenib. I Bar graphs showing the quantification of the frequencies of cells in individual cell cycle phases. Data are expressed as mean ± SEM. **p ≤ 0.001 (Student’s test, two-sided)