Fig. 2
RUNX1 promotes CCL2-mediated recruitment of TAMs. A Correlation between the RUNX1 expression and chemokine family members in 33 TCGA tumors. B Heat map analysis of the chemokines and its receptors based on the RNA-Seq data of HCT116 RUNX1OE/vector and SW480 shRUNX1/scramble. C Enrichment analysis of RUNX1-related signaling pathways using Wikipathway Cancer platform. D Correlation analysis between the RUNX1 mRNA and CCL2 mRNA in tumor specimens (n = 30). E Correlation analysis between RUNX1 IHC staining and CCL2 staining in 12 pairs of CRC samples. F, G RT-qPCR analysis of the expression of CCL2 mRNA in stably transfected CRC cells. H, I ELISA analysis of the CCL2 generation in supernatant of RUNX1 over-expressed or knockdown CRC cells. J (left) Representative IF staining of CCL2 in the orthotopic CRC tumors with RUNX1 over-expressed. Scale bars: 200 μm. (right) Quantification of percentage of CCL2+ HCT116 cells (of HCT116 cells; n = 3). Quantitative data are indicated as mean ± SEM. K (left) Possible binding sites of RUNX1 in the CCL2 core promoter. (right) ChIP-qPCR assay in HCT116. L All PCR products were confirmed by DNA agarose gel electrophoresis. M Dual-Glo luciferase assay in HCT116 and SW480