Fig. 6
From: LINC01559 promotes lung adenocarcinoma metastasis by disrupting the ubiquitination of vimentin
LINC01559 stabilizes vimentin. A Knockdown of LINC01559 accelerated the turnover of the vimentin protein in A549 and H1299 cells as shown in cycloheximide (CHX)-chase assays. n = 3 independent experiments. B Treatment with MG132 diminished the reductions in the vimentin protein caused by LINC01559 knockdown in A549 and H1299 cells. n = 3 independent experiments. C and D SiRNA knockdown of LINC01559 increased (C), whereas LINC01559 overexpression decreased (D) vimentin polyubiquitination in A549 and H1299 cells. n = 3 independent experiments. E A549.shLINC01559 lung metastatic tumors exhibited stronger vimentin polyubiquitination compared with lung metastatic tumors formed by A549.sh-ctrl cells. n = 5 biologically independent lung metastatic tumors