Fig. 5

LINC01559 promotes vimentin expression. A SiRNA knockdown of LINC01559 did not affect the levels of vimentin mRNA in A549 and H1299 cells. Data are mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparisons test. B SiRNA knockdown of LINC01559 reduced vimentin protein expression in A549 and H1299 cells. n = 3 independent experiments. C and D Overexpression of LINC01559 upregulated vimentin at the protein level (C), but did not alter vimentin mRNA expression (D). Data are mean ± s.d. (D); n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparisons test. E and F SiRNA knockdown or overexpression of vimentin did not cause any significant changes in the levels of LINC01559 expression in in A549 and H1299 cells. Data are mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparisons test. G A549.shLINC01559 lung metastatic tumors exhibited reduced levels of the vimentin protein compared with lung metastatic tumors formed by A549.sh-ctrl cells. n = 5 biologically independent lung metastatic tumors