Fig. 4

Vimentin is necessary for LINC01559-mediated LUAD cell migration and invasion. A-C SiRNA knockdown of vimentin (A) reduced migration and invasion (B, C) of A549 and H1299 cells. Scale bars 200 μm. The numbers of migrated and invasive cells were quantitated using the ImageJ software. Data are mean ± s.d. (A and C); n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparisons test. D and E Overexpression of vimentin prevented the reductions in migration and invasion caused by siRNA knockdown of LINC01559 in A549 and H1299 cells. The numbers of migrated and invasive cells were quantitated using the ImageJ software. Scale bars 200μm. Data are mean ± s.d. (E); n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparisons test. F SiRNA knockdown of vimentin diminished the increases of cell migration and invasion caused by LINC01559 overexpression in A549 and H1299 cells. Scale bars 200μm. Data are mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparisons test