Fig. 3
From: LINC01559 promotes lung adenocarcinoma metastasis by disrupting the ubiquitination of vimentin
LINC01559 binds to vimentin. A qPCR analysis showing LINC01559 is predominantly located to the cytoplasm. Analysis of β-actin was included as a cytoplasmic marker, and U1, a nuclear marker. Data are mean ± s.d.; n = 3 independent experiments. B RNA pulldown followed by mass spectrometry analysis identified that vimentin was the most abundant protein co-pulled down with LINC01559. S: sense; AS: antisense. n = 1 experiment. C Vimentin was co-pulled down with LINC01559 in A549 and H1299 cells as shown in RNA pulldown assays. RPD, RNA pulldown. n = 3 independent experiments. D LINC01559 was co-precipitated with vimentin in A549 and H1299 cells as shown in RNA immunoprecipitation assays. RIP: RNA immunoprecipitation. n = 3 independent experiments