Fig. 2

LINC01559 promotes LUAD metastasis. A-E SiRNA knockdown of LINC01559 (A) inhibited wound healing (B, C) and migration and invasion (D, E) of A549 and H1299 cells. Wound width and the numbers of migrated and invasive cells were quantitated using the ImageJ software. Scale bars, 200 μm (wound healing assay) and 200 μm (cell migration and invasion assay). Data are mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparisons test. F and H Stable knockdown of LINC01559 using shRNA (F) inhibited A549 cell lung metastasis in vivo (G). Scale bars, 0.1 cm. The number of metastatic lesions was quantified (H) by counting lung nodules from lungs harvested from NOD/SCID mice that received tail vein injection of A549.sh-ctrl or A549.shLINC01559 cells. n = 6 biologically independent animals; Two-tailed Student’s t-test. I Representative photographs of Hematoxylin and Eosin (H&E) staining of lung tissues from NOD/SCID that received tail vein injection of A549.sh-ctrl or A549.shLINC01559 cells. Scale bar, 100 μm. Data are representatives of six biologically independent animals