Fig. 2

Exosomal-miR-3p promotes CLDN1 depended on enhancer elements. a The luciferase activity is increased when HEK293T cells co-transfecting with BKV-miR-3p and pGL3-CLDN1. The luciferase activity decreased once BKV-miR-3p and CLDN1 is mismatched. b An enrichment of H3K27ac in enhancer region containing BKV-miR-3p binding site exhibited by ChIP-qPCR. c An enrichment of H3K27ac in enhancer region containing BKV-miR-3p binding site exhibited by CUT&TAG sequencing. d Knockout of binding site by CRISPR/Cas9 fails to promote CLDN1 expression. e Representative images of lung sections stained for indicated markers. FITC + positive area is quantified as a measure of vascular permeability. BKV-inoculated cells enhance vascular leakage, which was counteracted if knockdown BKV-miR-3p and BKV-miR-5p. f H&E staining of Liver sections. Area 1 in BKV group with positive expression of LTAg indicates that tumor cells invade blood vessel. g Statistics of liver metastasis in Con, BKV and anta-mir groups