Fig. 1

p-FAK was upregulated in crizotinib-resistant TNBC cells. A The cell viability and IC50 of MDA-MB-231 parental cell line and crizotinib-resistant line (MDA-MB-231-R) following a 48-h incubation with various concentrations of crizotinib. B The cell viability and IC50 of the Hs578T parental cell line and crizotinib-resistant line (Hs578T-R) following a 48-h incubation with various concentrations of crizotinib. C, D Representative figures (left) and quantitative analysis (right) of colony formation assay of MDA-MB-231 and Hs578T parental cells, as well as crizotinib-resistant cells. E, F Representative figures (left) and quantitative analysis (right) of apoptosis measured by flow cytometry of MDA-MB-231 and Hs578T parental cells, as well as crizotinib-resistant cells. G, H, I, J Western blotting results of MDA-MB-231 and Hs578T parental cells, as well as crizotinib-resistant cells. K, L The p-FAK levels between parental and crizotinib-resistant cells. Data are represented as mean ± SEM. Significant levels: ns = not significant (P > 0.05), * P < 0.05, ** P < 0.01, and *** P < 0.001