Fig. 8
From: Long-read transcriptome landscapes of primary and metastatic liver cancers at transcript resolution
Altered transcriptome profiles and characteristics of the liver in metastatic liver cancer. a. Comparison of the number of novel transcripts to annotated GENCODE transcripts for genes related to glycine, serine and threonine metabolism. b. Global transcript expression patterns of liver samples depicted through a t-SNE projection, including LM-NT samples and GTEx normal liver samples. c. Pathway enrichment analysis of genes associated with DETs in LM-NT samples. d. The difference in relative abundance of infiltrating immune cells between LM-NT and GTEx normal liver samples using the CIBERSORT algorithm. A difference > 0 indicates the enrichment of immune cells in LM-NT, and the color of the column represents the statistical significance of the difference. e-f. Changes in mRNA expression of chemokines, cytokines, and their receptors in LM-NT compared to GTEx normal liver samples. g. Change in mRNA expression of major histocompatibility complex (MHC) molecules, costimulators, and coinhibitors in LM-NT compared to GTEx normal liver samples. h-i. Representative IHC images displaying the expression of PD-1 and PD-L1 in LM-NT and hemangioma liver tissues. Scale bars, 100 μm or 50 μm