Fig. 4

Landscape of isoform switching events in primary liver cancer. a. Comparison of the average number of transcripts per gene for each item in Fig. 2c. P-values for the abundance of transcripts per gene in DET-specific and DEG-specific genes were calculated using the Wilcoxon rank-sum test. b. Percentage of genes with multiple TSSs for each item. P-values for the enrichment of genes with multiple TSS in DET-specific and DEG-specific genes were calculated using a chi-squared test. c. Pathway enrichment analysis for gene associated with isoform switch transcripts. d. Structure of the BANP and EMP2 transcripts in HCC and HCC-NT. e. K-means clustering of the isoform switch ratio. f. Kaplan-Meier curves illustrating overall survival in different isoform switch cluster patterns in the TCGA-LIHC cohort. Statistical significance was determined using Log-rank tests. Hazard ratio (HR) was calculated using the Cox proportional hazards regression model. g. Differences in the expression of immune checkpoint inhibitors among different types of isoform switch cluster patterns. ***p < 0.001, n.s., not significant