Fig. 9
From: S100A8/A9 as a risk factor for breast cancer negatively regulated by DACH1
The regulation effect of DACH1 on S100A8/A9 expression. (A–H) Scatter plots showing the correlations between S100A8/A9 and DACH1 mRNA levels using four datasets. (I) Western blotting assays showing the DACH1 protein level in CAMA-1 and MDA-MB-231 cells. (J) ELISA demonstrated that DACH1 inhibited S100A8 expression in CAMA-1 and MDA-MB-231 cells. (K) The representative IHC staining images showing the expression levels of S100A8, S100A9, and DACH1 in the same sample. (L) Heatmap showing the IHC scores of S100A8, S100A9, and DACH1 in IHC staining assays. (M–N) Scatter plots showing the correlations between S100A8/A9 and DACH1 protein levels using IHC scores. (O) The blend Kaplan-Meier curves of S100A8/A9 and DACH1 with OS of breast cancer patients of tissue microarrays. OS: overall survival; IHC: immunohistochemical