MiR-151a: a robust endogenous control for normalizing small extracellular vesicle cargo in human cancer

Biomarker Research

Table 1 List of the nine potential endogenous miRNAs identified by small RNA seq. In addition, the gold standard miR-16 normalizer and an external miRNA (miR-451) were used for normalizing purposes

MATURE-ID	Accession Number	Length (pb)	Mean Internal Variability	Mean Internal Variability in ovarian cancer samples	Mean Internal Variability in lung cancer samples	TPM per sample	Log2(Mean Expression) Variance in 30 cancer types (TCGA)
MATURE-ID	Accession Number	Length (pb)	(\|log2FC RvsS\|)	(\|log2FC RvsS\|)	(\|log2FC RvsS\|)	TPM per sample	Log2(Mean Expression) Variance in 30 cancer types (TCGA)
hsa-miR-151a-3p	MI0000809	21	0.084	0.105	0.041	6759	0.32
hsa-miR-22-5p	MI0000078	22	0.169	0.066	0.376	39.33	0.58
hsa-miR-502-3p	MI0003186	22	0.193	0.263	0.052	39.5	0.61
hsa-miR-221-3p	MI0000298	23	0.257	0.356	0.054	851.67	1.67
hsa-miR-1183	MI0006276	27	0.276	0.35	0.129	112.5	NA
hsa-miR-27a-3p	MI0000085	21	0.278	0.296	0.242	747.67	6.67
hsa-let-7i-5p	MI0000434	22	0.297	0.441	0.011	9586.5	0.6
hsa-miR-411-5p	MI0003675	21	0.323	0.196	0.579	54.17	3.05
hsa-miR-196b-5p	MI0001150	22	0.329	0.218	0.55	87	3.67
hsa-miR-16-5p	MI0000070	22	0.477	0.347	0.737	103.34	0.58
hsa-miR-451a	MI0001729	22	3.014	3.37	2.304	8884.5	1.24

R Tumor cells resistant to platinum-based chemotherapy treatment, S Tumor cells sensitive to platinum-based chemotherapy treatment, TPM Transcripts per kilobase million, TCGA Tumor Cancer Genome Atlas

ISSN: 2050-7771