Fig. 2

Characteristics of ScfDNA. ScfDNA insert size profile with multiple peaks and valleys using (A) Double-stranded library preparation (Peach solid line) and single-stranded library preparation (Turquoise solid line). (B) Single-stranded library preparation (Turquoise solid line) and Nick repair enzyme-treated ScfDNA (Turquoise dashed line). (C) Double-stranded library preparation (Peach solid line) and nick repair enzyme-treated ScfDNA (Peach dashed line). (D) Human and microbial origins of salivary cell-free DNA. Percentage of reads of ScfDNA mapping to humans and microbes. (E) Genomic element analysis using different library preparation methodologies is comparable to each other. Representative data obtained from single healthy donor