Table 5 Advantages and disadvantages of lectin-based detection approaches in cancer biomarker discovery
From: Lectins as potential tools for cancer biomarker discovery from extracellular vesicles
Tools used | Advantages | Disadvantages |
|---|---|---|
Lectin arrays | ▪ rapid and highly sensitive ▪ allow high throughput screening ▪ allow multiplexing ▪ do not require sample preparation ▪ use small amount of samples ▪ unbiased binding to glycans | ▪ non-specific binding may happen ▪ need extensive optimization |
Lectin blotting | ▪ easy to use ▪ reproducible ▪ highly sensitive and specific ▪ suitable for complex protein samples ▪ visualize small amounts of antigens | ▪ membrane may affect chemical stability of lectins ▪ non-specific binding may happen |
Lectin affinity chromatography | ▪ produce pure analyte ▪ high affinity ▪ do not require sample preparation ▪ do not need purified glycans | ▪ may co-elute other proteins ▪ need large amounts of samples ▪ non-specific binding may happen ▪ time-consuming |
Lectin-binding assay | ▪ simple and rapid ▪ very cost effective ▪ easy to perform ▪ requires minute amounts of samples ▪ high sensitivity and specificity | ▪ purified glycan may require as a standard ▪ non-specific binding may happen |