Fig. 1

Comparison of Musashi sequences, structure and expression profile. (A) Alignment of MSI-1 and MSI-2 performed using www.ebi.ac.uk [38]. Green colored amino acids are identical between MSI-1 and MSI-2 and blue colored amino acids indicate strongly similar properties. Grey amino acids indicate weakly similar properties. The total sequence identity is 68,8% between the two proteins. The two RNA binding motifs are underlined in black and red, respectively. Figure was modified based on Lan et al. 2020 [19]. (B) Crystal structure of the human RBD 1 of MSI-2, in a resolution of 2.1 Å. Structure based on the PDB-data 6NTY by Lan et al. 2020 [19]. Both RBD (RBD1 is shown here) are formed by beta-strand parts and alpha helical domains. The color gradient follows the structure from blue (N-terminal) to red (C-terminal). (C) Protein expression profile for different tissues based on the dataset of the human protein atlas webpage [24,25,26,27,28]. Tissues in which MSI-1 (black) was detectable were selected and MSI-2 is shown in red as comparison. MSI-2 was expressed in more tissues as shown in this graph. Annotation of the expression level was based on immunohistochemically stained tissues. The specific process is detailed in the webpage. (D) MSI-1 and MSI-2 RNA expression profile in the ten strongest-expressing tissues. Data were taken from the database the human protein atlas [24,25,26,27,28]. The normalized expression (nTPM) is created by the combination of HPA and GTEx transcriptomics datasets. Detailed information about data collection and normalization are available on the webpage