Fig. 6

Schematic representation of SOMAScan. (1) The labeled SOMAmers are incubated with prepared samples to bind with proteins, and then the binding complexes are tagged with biotin (B) and fluorescent label (F); (2)The avidin beads (AB) are used to capture SOMAmers-protein complexes; (3)The biotin need to tag to the proteins; (4)The ultraviolet light (hv) is utilized to cleave the photocleavable group in order to release the complexes; (5) Polyanionic competitors (PC) are introduced to remove the nonspecific SOMAmers; (6)The primer bead (PB) facilitate the complexes capture; (7) Captured complexes are dissociated in specific high pH condition