Skip to main content
Fig. 4 | Biomarker Research

Fig. 4

From: ICOS DNA methylation regulates melanoma cell-intrinsic ICOS expression, is associated with melanoma differentiation, prognosis, and predicts response to immune checkpoint blockade

Fig. 4

ICOS DNA methylation correlates with ICOS mRNA expression, immune cell infiltration, interferon γ, and tumoral differentiation in melanoma. A Genomic organization of the ICOS gene locus (ICOS transcripts, regulatory elements, guanosine/cytosine (GC)-density). Target sites of the Infinium BeadChip beads (CpG sites 1–7), qMSP (CpG site 4/5), and qRT-PCR are depicted. The illustration (modified) was exported from www.ensemble.org (Ensembl Release 109) and is based on Genome Reference Consortium Human Build 38 patch release 13 (GRCh38.p13). B Spearman’s correlations (Spearman’s ρ) of ICOS mRNA expression and ICOS DNA methylation levels obtained from the TCGA melanoma cohort and The Genomics of Drug Sensitivity in Cancer (GDSC) database (N = 33 melanoma cell lines; [30]; https://www.cancerrxgene.org/). Further, ICOS mRNA expression and ICOS DNA methylation status in the TCGA melanoma cohort was correlated with the lymphocyte score according to TCGA, with interferon γ (IFNG) mRNA, RNA-Seq signatures of immune cell infiltrates (CD8+ T cells: CD8A and CD8B, CD4.+ T cells: CD4, B cells: CD19 and CD20, monocytes: CD14), and with melanoma differentiation based on MITF and AXL mRNA expression. *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page

Biomarker Research

ISSN: 2050-7771

Contact us