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Fig. 3 | Biomarker Research

Fig. 3

From: ICOS DNA methylation regulates melanoma cell-intrinsic ICOS expression, is associated with melanoma differentiation, prognosis, and predicts response to immune checkpoint blockade

Fig. 3

Pharmacological demethylation induces ICOS expression in melanoma cells. A Normalized histograms illustrating induction of ICOS expression in pharmacologically demethylated (5-aza-dC treated) compared to untreated A375 melanoma cell line (unstained, anti-ICOS, and isotype control). B ICOS expression (mean fluorescence intensity [MFI]) over isotype control of 5-aza-dC treated compared to untreated A375 melanoma cells (six replicates). C ICOS mRNA (ΔCT levels) expression in 5-aza-dC treated compared to untreated A375 melanoma cells (six replicates) and six individual tonsils. D Representative ICOS IHC of 5-aza-dC treated compared to untreated A375 melanoma cells (40 × magnification). Bars represent mean values. P values refer to paired t-tests (*P < 0.05; ***P < 0.001)

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Biomarker Research

ISSN: 2050-7771

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