Fig. 6

Knockdown of KNTC1 inhibits CLL and attenuates the effects of SYT7 overexpression. A The knockdown efficiency of KNTC1 by shKNTC1 in MEC-2 cells was assessed by qPCR. B, C The mRNA and protein expression of SYT7 and KNTC1 was detected in NC(OE + KD) group and SYT7 + shKNTC1 group of MEC-2 cells by qPCR (B) and western blotting (C), respectively. D CCK8 assay was utilized to show the effects of mere KNTC1 knockdown or simultaneous SYT7 overexpression and KNTC1 knockdown on MEC-2 cell proliferation. E, F Flow cytometry was utilized to show the effects of mere KNTC1 knockdown or simultaneous SYT7 overexpression and KNTC1 knockdown on MEC-2 cell apoptosis (E) and cell cycle distribution (F). G Transwell assay was performed to show the effects of mere KNTC1 knockdown or simultaneous SYT7 overexpression and KNTC1 knockdown on MEC-2 cell migration (scale bar = 400 μm). Data were shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, based on a Student’s T-Test