Fig. 5

Knockdown of SYT7 inhibits CLL through downregulating KNTC1. A The heatmap showed the differentially expressed genes between shSYT7 group and shCtrl group of MEC-2 cells identified by gene microarray. B The expression of KNTC1 in CLL tissues and corresponding tissues was detected by immunohistochemistry analysis (scale bar = 50 μm). C A chase experiment was performed to evaluate the protein stability of KNTC1 in MEC-2 cells with or without SYT7 knockdown, and cells with or without SYVN1 overexpression. D Western blotting was carried out to assess the regulatory effects of SYT7 or SYVN1 on KNTC1 with or without the treatment of proteasome inhibitor MG132. E An immunoprecipitation assay was used to visualize the change of ubiquitination of KNTC1 upon SYT7 knockdown or SYVN1 overexpression. F A co-immunoprecipitation assay was performed to verify the interaction between SYT7 and SYVN1