Fig. 1

Differential expression of miRNAs observed in tissue and salivary exosomes of OSCC patients. (a) Venn diagram representing the forty-seven common miRNAs that were differentially expressed in tissue and salivary exosomes derived from OSCC and leucoplakia patients at nominal adj p < 0.05. (b) Volcano plot depicting the twelve significantly expressed miRNAs in both our and TCGA datasets (p < 0.001). (c) Box plots depicting the differential expression patterns of seven miRNAs in larger cohorts of tumour tissue and salivary exosomes of OSCC and leucoplakia patients using real-time PCR (***p ≤ 0.001). U6 small RNA was used as internal control, and the relative mRNA levels were analysed using the ddCt method. Error bars represent mean ± SD of three independent experiments