Fig. 3
Circ_0001187 facilitates AML suppression by promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level. A AGO2 RIP assay using AGO2 antibody in THP-1 cells lysates. B-C The results of the GO enrichment and pathways analysis from RNA pull-down in THP-1 cells. D Western blot results of METTL3 in THP-1 and Molm-13 AML cells treated by sh-Circ compared with negative control. E The results of m.6A dot blot in THP-1 and Molm-13 cells treated by sh-Circ compared with negative control. F Western blot results of METTL3 in 293 T cell treated with MG132 (10 μM, 6 h) or chloroquine (100 μM, 6 h). G Western blot results of METTL3 in THP-1 cells with Circ_ 0001187 knockdown treated with 20 μg/ml CHX at different times. H Western blot results of METTL3 in THP-1 cells with Circ_ 0001187 knockdown or overexpression treated by DMSO or MG132 (10 μM, 10 h). I-J IP results of METTL3 ubiquitination in 293 T cells. K-M The effect of Circ_0001187 knockdown or Circ_0001187/ METTL3 inhibitor STM2457 on the proliferation or apoptosis of THP-1 and Molm-13 cells treated with Ara-c (1 μM, 24 h) or not. Data were analyzed using unpaired t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001