Fig. 2

Circ_0001187 suppresses AML progression in vitro and in vivo. A-B The effect of Circ_0001187 knockdown or overexpression on the proliferation of THP-1 and Molm-13 AML cells by CCK-8 assay. C, D The effect of Circ_0001187 knockdown or overexpression on the apoptosis of THP-1 and Molm-13 AML cells treated with Ara-c(1uM, 24 h) or not by flow cytometry. E THP-1 and Molm-13 AML cells were as sensitive to Circ_0001187 knockdown or overexpression in terms of cell differentiation. F Western blot results of P21, MDM2 and P53 in THP-1 cells with knockdown or overexpressed Circ_0001187. G-I The results of spleen and liver weight of Circ_0001187 knockdown AML mice. J Kaplan–Meier analysis of the overall survival of mice treated with sh-Circ or NC. K The results of GFP⁺ leukemia cell frequencies in spleen, bone marrow and peripheral blood of mice treated with sh-circRNA or NC. L H&E staining showing infiltration of leukemic cells in the spleen and liver of mice engrafted with sh-Circ AML cells compared with that in control mice. And the results of Ki67 staining in the spleen and liver of mice engrafted with sh-Circ AML cells. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001