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Fig. 6 | Biomarker Research

Fig. 6

From: LncRNA AC006064.4–201 serves as a novel molecular marker in alleviating cartilage senescence and protecting against osteoarthritis by destabilizing CDKN1B mRNA via interacting with PTBP1

Fig. 6

AC006064.4–201 (Gm49317-201) / PTBP1 / CDKN1B axis was conserved between human and mice. A Western blotting analysis of Mmp3, Mmp13, Sox9, Aggrecan, CDKN1B, p21 and p53 in MCs after treating with Gm49317-201 ASOs. B MRNA levels of Mmp3, Mmp13, Sox9, Aggrecan, CDKN1B, p21 and p53 assessed by qRT-PCR in MCs after treating with Gm49319 ASOs (n = 9, 3 donors for three replicates) ** p < 0.01, *** p < 0.001. C Representative images and quantification of β-galactosidase staining in the above three groups. ** p < 0.01. D RNA–protein colocalization assay confirmed the interaction between Gm49317-201 and PTBP1 in MCs. Scale bar, 25 µm. E Western blotting analysis of Mmp3, Mmp13, Sox9, Aggrecan, CDKN1B, p21 and p53 in MCs after knocking down of CDKN1B. F MRNA levels of Mmp3, Mmp13, Sox9, Aggrecan, CDKN1B, p21 and p53 assessed by qRT-PCR in MCs after knocking down of CDKN1B (n = 9, 3 donors for three replicates) * p < 0.05, ** p < 0.01, *** p < 0.001. G Representative images and quantification of β-galactosidase staining of MCs after knocking down of CDKN1B. ** p < 0.01. H Western blotting analysis of Mmp3, Mmp13, Sox9, Aggrecan, CDKN1B, p21 and p53 in MCs after co-upregulation of Gm49317-201 and CDKN1B. I MRNA levels of Mmp3, Mmp13, Sox9, Aggrecan, CDKN1B, p21 and p53 assessed by qRT-PCR in MCs after co-upregulation of Gm49317-201 and CDKN1B (n = 9, 3 donors for three replicates) * p < 0.05, ** p < 0.01, *** p < 0.001. J Representative images and quantification of β-galactosidase staining in the above three groups. * p < 0.05, ** p < 0.01

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Biomarker Research

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