Fig. 4

ITPA is a functionally important target of ALKBH5. A The mRNA level of ITPA upon ALKBH5 knockdown in Kasumi-1 cells, primary AML cells, and murine RR/KIT cells, respectively. B The representative WB images of ALKBH5 and ITPA upon ALKBH5 knockdown in Kasumi-1 cells, primary AML cells, and murine RR/KIT cells, respectively. C Western blots of ALKBH5 and ITPA in Kasumi-1 cells after transduced with shNC or shALKBH5, together with an empty (vector) or ITPA-encoding lentivirus as indicated. After drug selection, those co-transduced cells were prepared for cell proliferation assays (D) or colony-forming assays (E)