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Fig. 3 | Biomarker Research

Fig. 3

From: Circular RNA circEMB promotes osteosarcoma progression and metastasis by sponging miR-3184-5p and regulating EGFR expression

Fig. 3

CircEMB promotes OSA cell proliferation, migration, invasion, apoptosis and cell cycle distribution. a Two shRNAs were designed to silence circEMB expression without affecting the mRNA expression of EMB. sh-circEMB #1 had the highest silencing efficiency. b CCK-8 assay revealed that circEMB knockdown inhibited the proliferation of 143B and U2OS cells. c Colony formation assay revealed that circEMB knockdown inhibited the proliferation of 143B and U2OS cells. d Transwell assay was performed to assess the influence of circEMB knockdown on the invasion and migration capabilities of 143B and U2OS cells. Scale bar, 100 μm. e Wound-healing assay was used to assess the effects of circEMB knockdown on 143B and U2OS cell migration. Scale bar, 200 μm. f Effects of circEMB silencing on apoptosis were examined via APC annexin V/PI staining as well as flow cytometry. g Flow cytometry was performed to assess the effects of circEMB on the cell cycle of 143B and U2OS cells. Data are expressed as mean ± SD (n = 3) (*, p < 0.05; **, p < 0.01; ***, p < 0.001)

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Biomarker Research

ISSN: 2050-7771

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