Fig. 4

miR-124-3p inhibits tumor metastasis by targeting Rab27a. a Sequence alignment of miR-12-3p with WT-Rab27a-3′-UTR and MUT- Rab27a-3′-UTR. The mutant sites were colored in red. b Luciferase activity from cells transfected with miR-124-3p and luciferase reporter constructs containing the WT-3-UTR or MUT-3-UTR of Rab27a. c qPCR to detect expression of Rab27a in A549 and NCI-H1299 cells transfected with miR-124-3p mimic, inhibitor, or mimic NC (control). d Western blot to detect protein expression of Rab27a in A549 and NCI-H1299 cells transfected with miR-124-3p mimic, inhibitor or mimic NC (control). Full-length blots are presented in Additional file 3. e Exosome number in medium from A549 and NCI-H1299 cells transfected with siRNA against Rab27a, si-Rab27a. f Transwell assays performed on A549 and NCI-H1299 cells transfected with si-Rab27a. g Transwell assays performed in A549 cells transfected with miR-124-3p mimic or miR-124-3p mimic + oe-Rab27a. h Transwell assays performed in NCI-H1299 cells transfected with miR-124-3p mimic or miR-124-3p mimic + oe-Rab27a. Data are shown as the mean ± SD. *P < 0.01, **P < 0.001, ***P < 0.0001, and ****P < 0.00001