Fig. 7

Inhibition of SLC25A21-AS1 and reversal of PTBP3 in xenograft tumor models. a SKOV3 cells stably expressing SLC25A21-AS1, both SLC25A21-AS1 and PTBP3 or empty vectors were subcutaneously injected into nude mice, each mouse was injected with 1x107 cells, 35 days later, the nude mice were euthanized and the tumors were completely removed and photographed. b Tumor volume (ab2/2) was measured every 6 days from seeding of stably expressing cells. c Tumor weight on the day after euthanizing nude mice. Datas represent mean ± SEM (n=7). d H&E staining and immunohistochemistry (IHC) were used to detect the proliferation, apoptosis and relative protein levels of PTBP3 in different groups. Ki67 shows proliferation levels and TUNEL shows apoptosis. Scale bars=100um. e Using an in vivo bioluminescence imaging system to detect tumor metastasis in different groups. Representative images (n=7) are shown