Fig. 3

SGOL2 dysregulated the cell cycle by regulating MAD2 in HCC cells. A SK-HEP-1 and HEP3B cells were transfected with shNC or shSGOL2, and the levels of PCNA, cyclin D1, cyclin E1, SGOL2, and MAD2 were detected by Western blots to study the effect of SGOL2 on cell cycle and MAD2. GAPDH was used as a loading control. B SK-HEP-1, and HEP3B cells were transfected with SGOL2 plasmid or vector control plasmid, and the levels of PCNA, cyclin D1, cyclin E1, SGOL2, and MAD2 were detected by Western blots. GAPDH was used as a loading control. C-D, Invasion and sphere formation of SGOL2-upregulated HCC cells with or without M2I-1 treatment were detected and analyzed. The results are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001