Fig. 6
Knockdown of METTL1 selectively inhibits translation of oncogenic mRNAs. A TE Scatter plot in METTL1 knockdown and control NBL cells. B Frequency of m7G tRNA decoding codons for increased TEs (TE-up), decreased TEs (TE-downdown), and unaltered TEs (TE-non). C Venn diagram of genes with decreased TEs in KELLY and BE2C cells. D Pathway enrichment of TE-down genes. E mRNA levels of MTDH and PDCD10. F RNC-qPCR confirmed translational downregulation of MTDH and PDCD10 after METTL1 knockdown. G Western blot confirmed the decreased protein levels of MTDH and PDCD10 in METTL1 knockdown NBL cells. Data are presented as Mean ± SD. P values were calculated by One-way ANOVA with Dunnett’s multiple comparisons test for (B, compared to TE-Down group), (E-F, compared to the shGFP group). **p < 0.01, ****p < 0.0001