Skip to main content
Fig. 5 | Biomarker Research

Fig. 5

From: METTL1 promotes neuroblastoma development through m7G tRNA modification and selective oncogenic gene translation

Fig. 5

METTL1 regulates tRNA m7G modification, tRNA expression and global mRNA translation in NBL cells. A Northwestern blot confirmed decreased levels of m7G modification in METTL1-knockdown NBL cells (KELLY shM1–1). U6 snRNA was used as a loading control. B A total of 21 m7G -modified tRNAs were identified by TRAC-seq. C Motif sequences at the tRNA m7G site. D Quantification of m7G-modified tRNAs in METTL1 knockdown and control cells (KELLY shM1–1 and KELLY shNC). E Knockdown of METTL1 in NBL cells resulted in reduced expression of m7G -modified tRNAs. F Polysome profiling of METTL1 knockdown and control NBL cells. G Puromycin intake assay of METTL1-inhibited and control KELLY cells. Data are expressed as Mean ± SD. P values were calculated by Mann-Whitney U test

Back to article page

Biomarker Research

ISSN: 2050-7771

Contact us