Fig. 5
From: Long noncoding RNA HOXC-AS3 interacts with CDK2 to promote proliferation in hepatocellular carcinoma
HOXC-AS3 correlates with CDK2-mediated cell cycle progression. A Total lysates from HEK293T cells expressing HA-p21 and Flag-CDK2 in the presence of HOXC-AS3 were subjected to IP with HA Ab, followed by western blotting using the indicated Abs. GAPDH, HA-P21 and Flag-CDK2 were used as a loading control. B Total lysates from HLF cells in the presence of HOXC-AS3 were subjected to IP with CDK2 Ab, followed by western blotting using the indicated Abs. GAPDH, p21 and CDK2 were used as a loading control. C RIP assay to confirm the association between HOXC-AS3 and P21 in HLF and 97H cells. D RNA pull-down assay to confirm the association between HOXC-AS3 and P21. E Western blot analysis of CDK2, Rb, and phosphorylated Rb in controls, oe-HOXC-AS3, siNC and Aso-HOXC-AS3 groups. F Western blot analysis of CDK2 and p21 with siNC, Aso-HOXC-AS3, Aso-HOXC-AS3 + pcDNA-vec and Aso-HOXC-AS3 + pcDNA-CDK2 groups in HLF cells. Western blot analysis of CDK2 and p21 with oe-vec, oe-HOXC-AS3, oe-HOXC-AS3 + siNC and oe-HOXC-AS3 + siCDK2 groups in Hep3B cells. Data represent the MEAN ± S.E.M of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (Student’s t test)