Fig. 2

High expression of TRAF3IP2 induces development of NONO-TFE3 tRCC. A-E The effects of TRAF3IP2 overexpression or knockdown on the proliferation of UOK109 and 786-O cells respectively were examined by CCK-8 assay (A-B), colony formation assays (C-D) and tumor sphere formation (E). F EdU assays were used to detect the proliferation rate of UOK109 and 786-O cells after transfection for 48 h. G Cell cycle was analyzed using flow cytometry after transfection for 48 h. The statistical results of the proportion of cells in S stage in correspond groups. H Cell apoptosis was analyzed via flow cytometry using an Annexin V/PI kit after transfection for 48 h. I-K Migration and invasion assays were performed with transfected cells using Transwell inserts. L-M Nude mice were injected subcutaneously with 786-O cells and tumor formation monitored over a period of several weeks. N The tumor volume was measured as indicated. O Representative H&E staining of xenograft tumors. The data are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001