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Fig. 3 | Biomarker Research

Fig. 3

From: Bone morphogenetic protein 2 is a new molecular target linked to non-alcoholic fatty liver disease with potential value as non-invasive screening tool

Fig. 3

Palmitic acid overload upregulates mRNA levels of BMP2 in Huh7 hepatocytes. A Representative images of Oil Red O (ORO) staining and its quantification. B Hepatic BMP2 mRNA levels were determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold increase and presented as mean ± SEM relative to control condition [1]. C BMP2 levels in the cell supernatant determined by ELISA. Data are expressed as pg/ml and presented as mean ± SEM. Experimental conditions: Huh7 cells treated with 750 μM palmitic acid (PA750) for 16 h (n = 3 independent experiments performed by duplicate). **p < 0.01 and ***p < 0.005, PA750 vs. Control (C)

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Biomarker Research

ISSN: 2050-7771

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