Fig. 4

Evaluation of Clinical Correlations. A Swimmers’ plot of patients with baseline CTC samples, sorted by PFS. The legend indicates site(s) of metastases, asterisks indicate time of receipt of palliative radiation therapy, and arrows denote ongoing response to ICB. CPI denotes that the patient received single-agent checkpoint inhibitor therapy, and Co denotes that the patient received combination checkpoint inhibitor with chemotherapy (with exception of 1B, who received combination checkpoint inhibitor therapy with a targeted kinase inhibitor on a clinical trial). The heat map summarizes CTC parameters including enumeration of total CTCs, PD-L1-positive CTCs, and HLA I-positive CTCs. The mean fluorescence intensities of HLA I and PD-L1 expression per CTC are averaged in the heatmap. B Serial evaluation of CTC number and protein expression compared to radiographic assessment