Fig. 3

Evaluating the Precision of ESP Staining with Quantitative Microscopy with Cell Lines and Patient Samples. A Representative images of H358 cells with binary layers generated on Hoechst quantifying the expression of PD-L1 or HLA I. B Triplicate side-by-side aliquots of H358 and LNCaP cells processed with ESP technology on the ExtractMax demonstrate the high repeatability precision of the automated cell staining and quantitative microscopy workflows. Each symbol represents the average logMFI value from each condition (approximately 3000 cells per condition). %CVs of replicates are annotated on graphs. C Cell lines harvested from three different flasks and evaluated on three different days demonstrates high reproducibility precision. Each symbol represents the average of replicate evaluations performed on three different days. D Representative images of HLA I and PD-L1 staining from a WBC and CTCs from a patient sample. E Replicate evaluations of patient samples demonstrate high precision for patients with many CTCs (left) and with few CTCs (right). Each symbol represents a single CTC, and each column represents a replicate evaluation of a patient sample. F High precision is demonstrated in many patient sample replicates. Each symbol represents the average logMFI of all CTCs within one replicate. The data represents 23 samples from 11 unique patients