Table 2 Characteristics of genomic DNA used as input for sequencing of tissue and brush swab biopsies
From: Brush swab as a noninvasive surrogate for tissue biopsies in epigenomic profiling of oral cancer
Sample | DNA concentration ng/μl | A260 | A280 | 260/280 | 260/230 | gDNA input, ng |
|---|---|---|---|---|---|---|
1C swab | 3.96 | 0.01 | −0.004 | −2.99 | −0.18 | 118.8 |
1C tissue | 22.00 | 4.45 | 2.210 | 2.01 | 2.18 | 660.0 |
1 N swab | 1.70 | −0.05 | −0.048 | 0.95 | 0.33 | 51.0 |
1 N tissue | 6.24 | 0.44 | 0.220 | 2.01 | 2.75 | 187.2 |
2C swab | 4.24 | 0.01 | −0.027 | −0.27 | −0.09 | 127.2 |
2C tissue | 11.40 | 1.00 | 0.463 | 2.16 | 2.85 | 342.0 |
2 N swab | 6.32 | 0.07 | 0.014 | 5.14 | −1.45 | 189.6 |
2 N tissue | 8.00 | 0.62 | 0.290 | 2.12 | 3.04 | 240.0 |
3C swab | 66.60 | 1.82 | 0.971 | 1.87 | 2.86 | 1998.0 |
3C tissue | 21.80 | 2.16 | 1.314 | 1.65 | 0.80 | 654.0 |
3 N swab | 23.60 | 0.45 | 0.217 | 2.06 | 4.70 | 708.0 |
3 N tissue | 8.48 | 0.39 | 0.191 | 2.05 | 5.66 | 254.4 |