Table 2 Technical characteristics of single cell genomic amplification methods
From: Application and prospects of single cell sequencing in tumors
| Â | Advantage | Disadvantage | Throughput | Ref |
|---|---|---|---|---|
DOP-PCR | Good uniformity | A large amount of sequence information is lost, a bias in amplification, low coverage | Low | [17] |
MDA | Simple, high coverage | A bias in amplification, may lead to gene fusion and allele loss. | Higher | [16] |
MALBAC | Good uniformity, high accuracy, good fidelity, both fresh and fixed single-cell samples can use | Efficiency is relatively low. | High | [16] |
LIANTI | High coverage, good uniformity, low error rate | High false positive rate of C-T base pairs | High | [14] |