Table 1 Main methods for isolating single cells
From: Application and prospects of single cell sequencing in tumors
Advantage | Disadvantage | Throughput | Effciency | Price | Ref | |
|---|---|---|---|---|---|---|
Limiting dilution | Simple | Time-consuming, easy to pollute | Low | Low | Low | [9] |
Microdroplets | convenient | High cost | high | high | high | [8] |
Micromanipulation | Visual, High success Rate | High technical requirements for operators, possible pollution | Low | Low | Low | [9] |
FACS | Wide application、Can sort tumor cells with complex molecular markers, Technical maturity, Standard unification | Damage to cells, requaire large initial cell count | High | High | Low | [10] |
Microfluidcis | High degree of automation, reduced pollutants, low sample consumption | High cost | High | High | High | [11] |
LCM | Spatiality fast | Easy to destory cells, accuracy is poor | low | low | High | [12] |