Table 1 A comparison between scFv and Nanobody in properties
From: A comprehensive comparison between camelid nanobodies and single chain variable fragments
Physiochemical properties | scFv | Nanobody |
|---|---|---|
size | 30-35 KDa 2×3 nm | 12-15 KDa 1×2.5 nm |
Half-life in blood | <1h | <<1h |
Preferred expression system | Bacteria | yeast |
Water solubility | + | +++ |
Aggregation | ++ | − |
Stability under harsh condition | + | +++ |
Tissue penetration | + | ++ |
Paratope diversity | + | ++ |
Cavity binding | − | + |
High affinity | + | + |
Ease of manipulation for enhance affinity | + | ++ |
Complexity of library construction techniques | ++ | + |
Nonspecific background binding | ++ | + |
Intracellular functionality | + | ++ |
Ease of expression | + | +++ |
Ability to concatenate | + | +++ |